Molecular evidence for a spotted fever group Rickettsia species in the tick Amblyomma longirostre in Brazil

Marcelo B. Labruna, Jere McBride, Donald Bouyer, Luis Marcelo A Camargo, Erney P. Camargo, David Walker

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

Two Amblyomma longirostre adult male ticks were collected from a Brazilian porcupine Coendou prehensilis L. in the state of Rondonia, Western Amazon, Brazil. The two ticks were pooled for DNA extraction and tested for the presence of rickettsial DNA by amplifying portions of the gltA, 17-kDa, ompA, and ompB rickettsial genes by polymerase chain reaction (PCR). Portions of the four genes were amplified from the sample and subsequently sequenced. These results indicated the presence of a Rickettsia strain infecting A. longirostre, which was designated as strain Aranha. Compared with homologous ompA rickettsial sequences, "Rickettsia amblyommii" ompA seemed to be the closest relative to Aranha (similarity values: 99.0-99.3%). Phylogenetic analyses of more conserved genes including 17-kDa and gtlA partial sequences indicated that this Rickettsia sp. is a spotted fever group rickettsia. The partial ompB sequence of strain Aranha was distinct from all homologous sequences available in GenBank. Although our ompA analysis suggested a very close molecular phylogenetic relationship of Aranha with "R. amblyommii," we cannot at this time determine if Aranha is a new strain of "R. amblyommii" or a new Rickettsia species in South America.

Original languageEnglish (US)
Pages (from-to)533-537
Number of pages5
JournalJournal of Medical Entomology
Volume41
Issue number3
StatePublished - May 2004

Fingerprint

Rickettsia
Amblyomma
Ticks
fever
Brazil
ticks
Fever
Porcupines
Genes
genes
South America
DNA
phylogeny
Nucleic Acid Databases
Sequence Homology
sequence homology
polymerase chain reaction
Polymerase Chain Reaction
sampling

Keywords

  • Amblyomma longirostre
  • Brazil
  • Rickettsia
  • Rondonia
  • Ticks

ASJC Scopus subject areas

  • Insect Science
  • veterinary(all)

Cite this

Molecular evidence for a spotted fever group Rickettsia species in the tick Amblyomma longirostre in Brazil. / Labruna, Marcelo B.; McBride, Jere; Bouyer, Donald; Camargo, Luis Marcelo A; Camargo, Erney P.; Walker, David.

In: Journal of Medical Entomology, Vol. 41, No. 3, 05.2004, p. 533-537.

Research output: Contribution to journalArticle

@article{2adb16996ce24311b368dd5c78006fb7,
title = "Molecular evidence for a spotted fever group Rickettsia species in the tick Amblyomma longirostre in Brazil",
abstract = "Two Amblyomma longirostre adult male ticks were collected from a Brazilian porcupine Coendou prehensilis L. in the state of Rondonia, Western Amazon, Brazil. The two ticks were pooled for DNA extraction and tested for the presence of rickettsial DNA by amplifying portions of the gltA, 17-kDa, ompA, and ompB rickettsial genes by polymerase chain reaction (PCR). Portions of the four genes were amplified from the sample and subsequently sequenced. These results indicated the presence of a Rickettsia strain infecting A. longirostre, which was designated as strain Aranha. Compared with homologous ompA rickettsial sequences, {"}Rickettsia amblyommii{"} ompA seemed to be the closest relative to Aranha (similarity values: 99.0-99.3{\%}). Phylogenetic analyses of more conserved genes including 17-kDa and gtlA partial sequences indicated that this Rickettsia sp. is a spotted fever group rickettsia. The partial ompB sequence of strain Aranha was distinct from all homologous sequences available in GenBank. Although our ompA analysis suggested a very close molecular phylogenetic relationship of Aranha with {"}R. amblyommii,{"} we cannot at this time determine if Aranha is a new strain of {"}R. amblyommii{"} or a new Rickettsia species in South America.",
keywords = "Amblyomma longirostre, Brazil, Rickettsia, Rondonia, Ticks",
author = "Labruna, {Marcelo B.} and Jere McBride and Donald Bouyer and Camargo, {Luis Marcelo A} and Camargo, {Erney P.} and David Walker",
year = "2004",
month = "5",
language = "English (US)",
volume = "41",
pages = "533--537",
journal = "Journal of Medical Entomology",
issn = "0022-2585",
publisher = "Entomological Society of America",
number = "3",

}

TY - JOUR

T1 - Molecular evidence for a spotted fever group Rickettsia species in the tick Amblyomma longirostre in Brazil

AU - Labruna, Marcelo B.

AU - McBride, Jere

AU - Bouyer, Donald

AU - Camargo, Luis Marcelo A

AU - Camargo, Erney P.

AU - Walker, David

PY - 2004/5

Y1 - 2004/5

N2 - Two Amblyomma longirostre adult male ticks were collected from a Brazilian porcupine Coendou prehensilis L. in the state of Rondonia, Western Amazon, Brazil. The two ticks were pooled for DNA extraction and tested for the presence of rickettsial DNA by amplifying portions of the gltA, 17-kDa, ompA, and ompB rickettsial genes by polymerase chain reaction (PCR). Portions of the four genes were amplified from the sample and subsequently sequenced. These results indicated the presence of a Rickettsia strain infecting A. longirostre, which was designated as strain Aranha. Compared with homologous ompA rickettsial sequences, "Rickettsia amblyommii" ompA seemed to be the closest relative to Aranha (similarity values: 99.0-99.3%). Phylogenetic analyses of more conserved genes including 17-kDa and gtlA partial sequences indicated that this Rickettsia sp. is a spotted fever group rickettsia. The partial ompB sequence of strain Aranha was distinct from all homologous sequences available in GenBank. Although our ompA analysis suggested a very close molecular phylogenetic relationship of Aranha with "R. amblyommii," we cannot at this time determine if Aranha is a new strain of "R. amblyommii" or a new Rickettsia species in South America.

AB - Two Amblyomma longirostre adult male ticks were collected from a Brazilian porcupine Coendou prehensilis L. in the state of Rondonia, Western Amazon, Brazil. The two ticks were pooled for DNA extraction and tested for the presence of rickettsial DNA by amplifying portions of the gltA, 17-kDa, ompA, and ompB rickettsial genes by polymerase chain reaction (PCR). Portions of the four genes were amplified from the sample and subsequently sequenced. These results indicated the presence of a Rickettsia strain infecting A. longirostre, which was designated as strain Aranha. Compared with homologous ompA rickettsial sequences, "Rickettsia amblyommii" ompA seemed to be the closest relative to Aranha (similarity values: 99.0-99.3%). Phylogenetic analyses of more conserved genes including 17-kDa and gtlA partial sequences indicated that this Rickettsia sp. is a spotted fever group rickettsia. The partial ompB sequence of strain Aranha was distinct from all homologous sequences available in GenBank. Although our ompA analysis suggested a very close molecular phylogenetic relationship of Aranha with "R. amblyommii," we cannot at this time determine if Aranha is a new strain of "R. amblyommii" or a new Rickettsia species in South America.

KW - Amblyomma longirostre

KW - Brazil

KW - Rickettsia

KW - Rondonia

KW - Ticks

UR - http://www.scopus.com/inward/record.url?scp=2542585496&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=2542585496&partnerID=8YFLogxK

M3 - Article

VL - 41

SP - 533

EP - 537

JO - Journal of Medical Entomology

JF - Journal of Medical Entomology

SN - 0022-2585

IS - 3

ER -