Molecular evidence for the origin of the widespread Venezuelan equine encephalitis epizootic of 1969 to 1972

R. M. Kinney, K. R. Tsuchiya, J. M. Sneider, D. W. Trent

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Venezuelan equine encephalitis (VEE) virus is a mosquito-borne pathogen that has caused encephalitis in equine species and humans during sporadic outbreaks in the western hemisphere. The last, and most widespread, VEE outbreak occurred in South America, Central America, Mexico and the U.S.A. (Texas) during 1969 to 1972. We have cloned and sequenced the genome of a virulent VEE subtype I-AB virus, strain 71-180, isolated in Texas in 1971. Thirty-four nucleotide differences were detected between the genome of 71-180 virus and that of the subtype I-AB Trinidad donkey (TRD) virus isolated during the 1943 VEE epizootic in Trinidad. Fifteen nucleotide changes occurred in the non-structural genes, 16 in the structural genes and three in the 3' non-coding region. Only six of the nucleotide differences resulted in amino acid substitutions: one change in each of non-structural proteins nsP1 and nsP3, two in the E2 envelope glycoprotein, one in the 6K polypeptide and one in the E1 envelope glycoprotein. The close genetic relationship between 71-180 virus and TRD virus, commonly used for production of formalin-inactivated VEE vaccines, suggests that incompletely inactivated virulent vaccine virus may have been the source of this and other VEE outbreaks. Use of formalized virulent virus was discontinued during the 1969 to 1972 panzootic. No VEE epizootics have been reported since the introduction of the live attenuated TC-83 vaccine

Original languageEnglish (US)
Pages (from-to)3301-3305
Number of pages5
JournalJournal of General Virology
Volume73
Issue number12
StatePublished - 1992

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Venezuelan Equine Encephalomyelitides
Viruses
Trinidad and Tobago
Disease Outbreaks
Nucleotides
Equidae
Equine Encephalomyelitis
Glycoproteins
Vaccines
Venezuelan Equine Encephalitis Viruses
Genome
Central America
Inactivated Vaccines
South America
Amino Acid Substitution
Mexico
Culicidae
Formaldehyde
Genes
Peptides

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Kinney, R. M., Tsuchiya, K. R., Sneider, J. M., & Trent, D. W. (1992). Molecular evidence for the origin of the widespread Venezuelan equine encephalitis epizootic of 1969 to 1972. Journal of General Virology, 73(12), 3301-3305.

Molecular evidence for the origin of the widespread Venezuelan equine encephalitis epizootic of 1969 to 1972. / Kinney, R. M.; Tsuchiya, K. R.; Sneider, J. M.; Trent, D. W.

In: Journal of General Virology, Vol. 73, No. 12, 1992, p. 3301-3305.

Research output: Contribution to journalArticle

Kinney, RM, Tsuchiya, KR, Sneider, JM & Trent, DW 1992, 'Molecular evidence for the origin of the widespread Venezuelan equine encephalitis epizootic of 1969 to 1972', Journal of General Virology, vol. 73, no. 12, pp. 3301-3305.
Kinney, R. M. ; Tsuchiya, K. R. ; Sneider, J. M. ; Trent, D. W. / Molecular evidence for the origin of the widespread Venezuelan equine encephalitis epizootic of 1969 to 1972. In: Journal of General Virology. 1992 ; Vol. 73, No. 12. pp. 3301-3305.
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