Molecular evolution and spatial transmission of severe fever with thrombocytopenia syndrome virus based on complete genome sequences

Jian Wei Liu, Li Zhao, Li Mei Luo, Miao Miao Liu, Yue Sun, Xiang Su, Xue Jie Yu

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Severe fever with thrombocytopenia syndrome virus (SFTSV) was a novel tick-borne bunyavirus that caused hemorrhagic fever with a high fatality rate in East Asia. In this study we analyzed the complete genome sequences of 122 SFTSV strains to determine the phylogeny, evolution and reassortment of the virus. We revealed that the evolutionary rate of three genome segments were different, with highest in the S segment and lowest in the L segment. The SFTSV strains were phylogenetically classified into 5 lineages (A, B, C, D and E) with each genome segment. SFTSV strains from China were classified in all 5 lineages, strains from South Korea were classified into 3 lineages (A, D, and E), and all strains from Japan were classified in only linage E. Using the average evolutionary rate of the three genome segments, we found that the extant SFTSV originated 20-87 years ago in the Dabie Mountain area in central China. The viruses were then transmitted to other areas of China, Japan and South Korea. We also found that six SFTSV strains were reassortants. Selection pressure analysis suggested that SFTSV was under purifying selection according to the four genes (RNA-dependent RNA polymerase, glycoprotein, nucleocapsid protein, non-structural protein), and two sites (37,1033) of glycoproteins were identified as being under strong positive selection. We concluded that SFTSV originated in central China and spread to other places recently and the virus was under purifying selection with high frequency of reassortment.

Original languageEnglish (US)
Article numbere0151677
JournalPLoS One
Volume11
Issue number3
DOIs
StatePublished - Mar 1 2016

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Molecular Evolution
thrombocytopenia
Viruses
Thrombocytopenia
fever
Fever
Genes
Genome
viruses
genome
China
Republic of Korea
South Korea
glycoproteins
Glycoproteins
Japan
RNA-directed RNA polymerase
Orthobunyavirus
nucleocapsid proteins
RNA Replicase

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Molecular evolution and spatial transmission of severe fever with thrombocytopenia syndrome virus based on complete genome sequences. / Liu, Jian Wei; Zhao, Li; Luo, Li Mei; Liu, Miao Miao; Sun, Yue; Su, Xiang; Yu, Xue Jie.

In: PLoS One, Vol. 11, No. 3, e0151677, 01.03.2016.

Research output: Contribution to journalArticle

Liu, Jian Wei ; Zhao, Li ; Luo, Li Mei ; Liu, Miao Miao ; Sun, Yue ; Su, Xiang ; Yu, Xue Jie. / Molecular evolution and spatial transmission of severe fever with thrombocytopenia syndrome virus based on complete genome sequences. In: PLoS One. 2016 ; Vol. 11, No. 3.
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