Molecular evolution of eastern equine encephalomyelitis virus in North America

Scott Weaver, Thomas W. Scott, Rebeca Rico-Hesse

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

We examined the rate and spatial pattern of eastern equine encephalomyelitis virus (EEEV) evolution in North America using primer-extension sequencing of viral RNA. Nucleotide sequences of the entire 26 S structural gene region of four EEEV strains revealed remarkable conservation between 1933 and 1985, with an estimated 0.7% divergence or 1.4 × 10-4 nucleotide substitutions per site per year. Sequences from smaller 26 S regions of nine additional strains suggested that EEEV evolves in North America in a single lineage, with genetic exchange regularly occurring among enzootic transmission foci. In these limited 26 S genome regions, only synonymous nucleotide substitutions became fixed between 1933 and 1988, implying a high degree of conservation in protein structure. Short nucleotide sequences from a Panamanian, South American variety isolate revealed a relatively distant relationship to North American serotype viruses. This suggested genetic divergence between antigenic varieties, and independent evolution of EEEV in North and South America. Factors related to replication and epidemiology of EEEV, which may constrain its evolution in nature, are discussed. Possible mechanisms of genetic exchange among enzootic foci are also considered.

Original languageEnglish (US)
Pages (from-to)774-784
Number of pages11
JournalVirology
Volume182
Issue number2
DOIs
StatePublished - 1991
Externally publishedYes

Fingerprint

Eastern equine encephalitis virus
Molecular Evolution
North America
Nucleotides
South America
Viral RNA
Epidemiology
Genome
Viruses
Genes
Proteins

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Molecular evolution of eastern equine encephalomyelitis virus in North America. / Weaver, Scott; Scott, Thomas W.; Rico-Hesse, Rebeca.

In: Virology, Vol. 182, No. 2, 1991, p. 774-784.

Research output: Contribution to journalArticle

Weaver, Scott ; Scott, Thomas W. ; Rico-Hesse, Rebeca. / Molecular evolution of eastern equine encephalomyelitis virus in North America. In: Virology. 1991 ; Vol. 182, No. 2. pp. 774-784.
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