Molecular genetic approaches to the analysis of neuropeptide receptors

J. F. Battey, Z. Fathi, E. Wada, Mark Hellmich, J. M. Way

Research output: Contribution to journalArticle

Abstract

Within the last few years, an increasing number of neuropeptide receptors have been structurally and functionally characterized. The analysis of these receptors, along with a precise understanding of their pharmacology and signal transduction pathways, has been facilitated by the use of molecular genetic techniques. This review will highlight some of the techniques currently being used to study neuropeptide receptors. Methods for isolation of complementary DNA clones for receptors, analysis of receptor messenger RNA expression and characterization of cloned receptors expressed in cultured cells are reviewed. In addition, a genetic approach for the analysis of neuropeptide receptor function in the context of a whole mammalian organism is discussed.

Original languageEnglish (US)
Pages (from-to)213-230
Number of pages18
JournalCellular Physiology and Biochemistry
Volume3
Issue number3-4
StatePublished - 1993
Externally publishedYes

Fingerprint

Neuropeptide Receptors
Molecular Biology
Genetic Techniques
Cultured Cells
Signal Transduction
Complementary DNA
Clone Cells
Pharmacology
Messenger RNA

ASJC Scopus subject areas

  • Cell Biology
  • Physiology

Cite this

Molecular genetic approaches to the analysis of neuropeptide receptors. / Battey, J. F.; Fathi, Z.; Wada, E.; Hellmich, Mark; Way, J. M.

In: Cellular Physiology and Biochemistry, Vol. 3, No. 3-4, 1993, p. 213-230.

Research output: Contribution to journalArticle

Battey, JF, Fathi, Z, Wada, E, Hellmich, M & Way, JM 1993, 'Molecular genetic approaches to the analysis of neuropeptide receptors', Cellular Physiology and Biochemistry, vol. 3, no. 3-4, pp. 213-230.
Battey, J. F. ; Fathi, Z. ; Wada, E. ; Hellmich, Mark ; Way, J. M. / Molecular genetic approaches to the analysis of neuropeptide receptors. In: Cellular Physiology and Biochemistry. 1993 ; Vol. 3, No. 3-4. pp. 213-230.
@article{1c0811afabb14752829ef4cff62fff92,
title = "Molecular genetic approaches to the analysis of neuropeptide receptors",
abstract = "Within the last few years, an increasing number of neuropeptide receptors have been structurally and functionally characterized. The analysis of these receptors, along with a precise understanding of their pharmacology and signal transduction pathways, has been facilitated by the use of molecular genetic techniques. This review will highlight some of the techniques currently being used to study neuropeptide receptors. Methods for isolation of complementary DNA clones for receptors, analysis of receptor messenger RNA expression and characterization of cloned receptors expressed in cultured cells are reviewed. In addition, a genetic approach for the analysis of neuropeptide receptor function in the context of a whole mammalian organism is discussed.",
author = "Battey, {J. F.} and Z. Fathi and E. Wada and Mark Hellmich and Way, {J. M.}",
year = "1993",
language = "English (US)",
volume = "3",
pages = "213--230",
journal = "Cellular Physiology and Biochemistry",
issn = "1015-8987",
publisher = "S. Karger AG",
number = "3-4",

}

TY - JOUR

T1 - Molecular genetic approaches to the analysis of neuropeptide receptors

AU - Battey, J. F.

AU - Fathi, Z.

AU - Wada, E.

AU - Hellmich, Mark

AU - Way, J. M.

PY - 1993

Y1 - 1993

N2 - Within the last few years, an increasing number of neuropeptide receptors have been structurally and functionally characterized. The analysis of these receptors, along with a precise understanding of their pharmacology and signal transduction pathways, has been facilitated by the use of molecular genetic techniques. This review will highlight some of the techniques currently being used to study neuropeptide receptors. Methods for isolation of complementary DNA clones for receptors, analysis of receptor messenger RNA expression and characterization of cloned receptors expressed in cultured cells are reviewed. In addition, a genetic approach for the analysis of neuropeptide receptor function in the context of a whole mammalian organism is discussed.

AB - Within the last few years, an increasing number of neuropeptide receptors have been structurally and functionally characterized. The analysis of these receptors, along with a precise understanding of their pharmacology and signal transduction pathways, has been facilitated by the use of molecular genetic techniques. This review will highlight some of the techniques currently being used to study neuropeptide receptors. Methods for isolation of complementary DNA clones for receptors, analysis of receptor messenger RNA expression and characterization of cloned receptors expressed in cultured cells are reviewed. In addition, a genetic approach for the analysis of neuropeptide receptor function in the context of a whole mammalian organism is discussed.

UR - http://www.scopus.com/inward/record.url?scp=0027226037&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027226037&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0027226037

VL - 3

SP - 213

EP - 230

JO - Cellular Physiology and Biochemistry

JF - Cellular Physiology and Biochemistry

SN - 1015-8987

IS - 3-4

ER -