Molecular identification of Rickettsia felis in ticks and fleas from an endemic area for Brazilian Spotted Fever

K. A. Oliveira, L. S. Oliveira, C. C A Dias, A. Silva, M. R. Almeida, G. Almada, Donald Bouyer, M. A M Galvão, C. L. Mafra

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Rickettsioses are arthropod-borne diseases caused by parasites from the Order Rickettsiales. The most prevalent rickettsial disease in Brazil is Brazilian Spotted Fever (BSF). This work intends the molecular detection of those agents in ectoparasites from an endemic area of BSF in the state of Espírito Santo. A total of 502 ectoparasites, among them Amblyomma cajennense, Amblyomma dubitatum (A. cooperi), Riphicephalus sanguineus, Anocentor nitens and Ctenocephalides felis, was collected from domestic animals and the environment and separated in 152 lots according to the origin. Rickettsia sp. was detected in pools of all collected species by amplification of 17kDa protein-encoding gene fragments. The products of PCR amplification of three samples were sequenced, and Rickettsia felis was identified in R. sanguineus and C. felis. These results confirm the presence of Rickettsia felis in areas previously known as endemic for BSF, disease caused by Rickettsia rickettsii. Moreover, they show the needing of further studies for deeper knowledge of R. felis-spotted fever epidemiology and differentiation of these diseases in Brazil.

Original languageEnglish (US)
Pages (from-to)191-194
Number of pages4
JournalMemorias do Instituto Oswaldo Cruz
Volume103
Issue number2
StatePublished - Mar 2008

Fingerprint

Rickettsia felis
Siphonaptera
Ticks
Fever
Ctenocephalides
Amplification
Brazil
Rickettsia rickettsii
Rickettsia
Epidemiology
Gene encoding
Arthropods
Domestic Animals
Parasites
Animals
Polymerase Chain Reaction
Proteins

Keywords

  • Brazilian spotted fever
  • Ctenocephalides felis
  • R. felis-spotted fever
  • Rickettsia felis
  • Rickettsia rickettsii
  • Tick

ASJC Scopus subject areas

  • Infectious Diseases
  • Virology
  • Immunology
  • Microbiology (medical)
  • Clinical Biochemistry

Cite this

Oliveira, K. A., Oliveira, L. S., Dias, C. C. A., Silva, A., Almeida, M. R., Almada, G., ... Mafra, C. L. (2008). Molecular identification of Rickettsia felis in ticks and fleas from an endemic area for Brazilian Spotted Fever. Memorias do Instituto Oswaldo Cruz, 103(2), 191-194.

Molecular identification of Rickettsia felis in ticks and fleas from an endemic area for Brazilian Spotted Fever. / Oliveira, K. A.; Oliveira, L. S.; Dias, C. C A; Silva, A.; Almeida, M. R.; Almada, G.; Bouyer, Donald; Galvão, M. A M; Mafra, C. L.

In: Memorias do Instituto Oswaldo Cruz, Vol. 103, No. 2, 03.2008, p. 191-194.

Research output: Contribution to journalArticle

Oliveira, KA, Oliveira, LS, Dias, CCA, Silva, A, Almeida, MR, Almada, G, Bouyer, D, Galvão, MAM & Mafra, CL 2008, 'Molecular identification of Rickettsia felis in ticks and fleas from an endemic area for Brazilian Spotted Fever', Memorias do Instituto Oswaldo Cruz, vol. 103, no. 2, pp. 191-194.
Oliveira KA, Oliveira LS, Dias CCA, Silva A, Almeida MR, Almada G et al. Molecular identification of Rickettsia felis in ticks and fleas from an endemic area for Brazilian Spotted Fever. Memorias do Instituto Oswaldo Cruz. 2008 Mar;103(2):191-194.
Oliveira, K. A. ; Oliveira, L. S. ; Dias, C. C A ; Silva, A. ; Almeida, M. R. ; Almada, G. ; Bouyer, Donald ; Galvão, M. A M ; Mafra, C. L. / Molecular identification of Rickettsia felis in ticks and fleas from an endemic area for Brazilian Spotted Fever. In: Memorias do Instituto Oswaldo Cruz. 2008 ; Vol. 103, No. 2. pp. 191-194.
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