Molecular mechanism of extinction of liver-specific functions in mouse hepatoma × rat fibroblast hybrids

Extinction of the albumin gene

John Papaconstantinou, Edith Wong, Harry Ratrie, Claude Szpirer, Josiane Szpirer

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Hybrids formed by the fusion of mouse hepatoma (BWTG3) and rat fibroblast (JF1) cells exhibit the extinction of mouse albumin and α-fetoprotein synthesis. Karyotype analyses suggest that all parental chromosomes are present in the hybrids. The extinction, therefore, of mouse hepatocyte genes is attributed to the inhibitory action of the rat genome. In these studies, we show that these hybrids possess and express the mouse β-glucuronidase gene (which is encoded on the same chromosome as the mouse albumin and α-fetoprotein gene), and we present data of Southern blot analysis which demonstrate that such hybrids have indeed retained both mouse and rat albumin DNA sequences. In addition, using mouse albumin cDNA, we have shown by cDNA-RNA reassociation kinetics that albumin mRNA is virtually absent in these hybrids. We conclude from these studies that the extinction of albumin synthesis involves a mechanism which results in the loss of cytoplasmic albumin mRNA.

Original languageEnglish (US)
Pages (from-to)363-376
Number of pages14
JournalSomatic Cell Genetics
Volume8
Issue number3
DOIs
StatePublished - May 1982
Externally publishedYes

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Albumins
Hepatocellular Carcinoma
Fibroblasts
Liver
Genes
Fetal Proteins
Complementary DNA
Chromosomes
Messenger RNA
Glucuronidase
Southern Blotting
Karyotype
Hepatocytes
Genome
RNA

ASJC Scopus subject areas

  • Genetics
  • Medicine(all)

Cite this

Molecular mechanism of extinction of liver-specific functions in mouse hepatoma × rat fibroblast hybrids : Extinction of the albumin gene. / Papaconstantinou, John; Wong, Edith; Ratrie, Harry; Szpirer, Claude; Szpirer, Josiane.

In: Somatic Cell Genetics, Vol. 8, No. 3, 05.1982, p. 363-376.

Research output: Contribution to journalArticle

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