Molecular mechanisms of extinction of liver-specific functions in mouse hepatoma rat fibroblast hybrids

Extinction of α-fetoprotein gene

Wesley K. Church, John Papaconstantinou, Sau Wah Kwan, Anne Poliard, Claude Szpirer, Josianne Szpirer

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Somatic cell hybrids formed by the fusion of mouse hepatoma (BWTG3) and rat fibroblast (JF1) cells exhibit the extinction of mouse AFP gene expression. Analysis of HindIII digests clearly shows that both mouse and rat AFP genes are present in the hybrids. DNA-RNA hybridization data show that AFP mRNA is virtually absent in the somatic cell hybrids. Our studies indicate, therefore, that extinction of mouse AFP gene expression in somatic cell hybrids (BJ01, BJ50, and BJ140) occurs in the presence of the rat genes and that the mechanism of extinction involves processes that result in the loss of AFP mRNA. We propose that the mechanism may involve either the inhibition of transcription or an inhibition of the processing of the mRNA transcript.

Original languageEnglish (US)
Pages (from-to)541-545
Number of pages5
JournalSomatic Cell and Molecular Genetics
Volume10
Issue number5
DOIs
StatePublished - Sep 1984

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Fetal Proteins
Hybrid Cells
Hepatocellular Carcinoma
Fibroblasts
Liver
Messenger RNA
Genes
Gene Expression
RNA
DNA

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Molecular mechanisms of extinction of liver-specific functions in mouse hepatoma rat fibroblast hybrids : Extinction of α-fetoprotein gene. / Church, Wesley K.; Papaconstantinou, John; Kwan, Sau Wah; Poliard, Anne; Szpirer, Claude; Szpirer, Josianne.

In: Somatic Cell and Molecular Genetics, Vol. 10, No. 5, 09.1984, p. 541-545.

Research output: Contribution to journalArticle

Church, Wesley K. ; Papaconstantinou, John ; Kwan, Sau Wah ; Poliard, Anne ; Szpirer, Claude ; Szpirer, Josianne. / Molecular mechanisms of extinction of liver-specific functions in mouse hepatoma rat fibroblast hybrids : Extinction of α-fetoprotein gene. In: Somatic Cell and Molecular Genetics. 1984 ; Vol. 10, No. 5. pp. 541-545.
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