Molecular recognition of watson–crick base‐pair reversals in triple‐helix formation: Use of nonnatural oligonucleotide bases

V. Mohan, Y. ‐K Cheng, Gail E. Marlow, B. Montgomery Pettitt

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

We report the calculated characteristics of nonnatural triplex‐forming oligonucleotide (TFO) bases recognizing base‐pair reversals (TA → AT) in a double‐helical DNA sequence. Ab initio and molecular mechanics calculations have been carried out to characterize the geometric and energetic consequences at the base‐pair reversal sites. We have estimated the free energies of solvation of the natural and proposed bases by solving the linearized Poisson–Boltzmann equation. The calculations indicate that the proposed TFO bases should bind with some specificity to the duplex. Implications of the strategy used in the context of molecular biology is discussed. © 1993 John Wiley & Sons, Inc.

Original languageEnglish (US)
Pages (from-to)1317-1325
Number of pages9
JournalBiopolymers
Volume33
Issue number9
DOIs
StatePublished - Sep 1993
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Biomaterials
  • Organic Chemistry

Fingerprint Dive into the research topics of 'Molecular recognition of watson–crick base‐pair reversals in triple‐helix formation: Use of nonnatural oligonucleotide bases'. Together they form a unique fingerprint.

  • Cite this