Molecular stretching modulates mechanosensing pathways

Xian Hu, Felix Martin Margadant, Mingxi Yao, Michael Sheetz

Research output: Contribution to journalReview article

16 Citations (Scopus)

Abstract

For individual cells in tissues to create the diverse forms of biological organisms, it is necessary that they must reliably sense and generate the correct forces over the correct distances and directions. There is considerable evidence that the mechanical aspects of the cellular microenvironment provide critical physical parameters to be sensed. How proteins sense forces and cellular geometry to create the correct morphology is not understood in detail but protein unfolding appears to be a major component in force and displacement sensing. Thus, the crystallographic structure of a protein domain provides only a starting point to then analyze what will be the effects of physiological forces through domain unfolding or catch-bond formation. In this review, we will discuss the recent studies of cytoskeletal and adhesion proteins that describe protein domain dynamics. Forces applied to proteins can activate or inhibit enzymes, increase or decrease protein-protein interactions, activate or inhibit protein substrates, induce catch bonds and regulate interactions with membranes or nucleic acids. Further, the dynamics of stretch-relaxation can average forces or movements to reliably regulate morphogenic movements. In the few cases where single molecule mechanics are studied under physiological conditions such as titin and talin, there are rapid cycles of stretch-relaxation that produce mechanosensing signals. Fortunately, the development of new single molecule and super-resolution imaging methods enable the analysis of single molecule mechanics in physiologically relevant conditions. Thus, we feel that stereotypical changes in cell and tissue shape involve mechanosensing that can be analyzed at the nanometer level to determine the molecular mechanisms involved.

Original languageEnglish (US)
Pages (from-to)1337-1351
Number of pages15
JournalProtein Science
Volume26
Issue number7
DOIs
StatePublished - Jul 1 2017
Externally publishedYes

Fingerprint

Stretching
Proteins
Mechanics
Organism Forms
Talin
Connectin
Cellular Microenvironment
Protein Unfolding
Cytoskeletal Proteins
Cell Shape
Molecules
Nucleic Acids
Tissue
Membranes
Enzymes
Adhesion
Imaging techniques
Geometry
Substrates
Protein Domains

Keywords

  • bioimaging
  • dSTORM
  • localization microscopy
  • mechanobiology
  • mechanoenzymatics
  • mechanosensing
  • molecular forces
  • protein stretching
  • single molecule

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Molecular stretching modulates mechanosensing pathways. / Hu, Xian; Margadant, Felix Martin; Yao, Mingxi; Sheetz, Michael.

In: Protein Science, Vol. 26, No. 7, 01.07.2017, p. 1337-1351.

Research output: Contribution to journalReview article

Hu, Xian ; Margadant, Felix Martin ; Yao, Mingxi ; Sheetz, Michael. / Molecular stretching modulates mechanosensing pathways. In: Protein Science. 2017 ; Vol. 26, No. 7. pp. 1337-1351.
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