Molluscan subtilisin-related endoproteases involved in the activation of prohormones and proproteins in the secretory pathway

Gregg T. Nagle, Susan L. Knock, Walter R A Van heumen, Alexander Kurosky

    Research output: Contribution to journalArticle

    1 Citation (Scopus)

    Abstract

    Neuropeptides and pcptide hormones are synthesized as part of larger precursor proteins that are processed post-translationally by subtilisin-related prohormone convertases (PCs), frequently at multiple basic sites, to generate biologically active peptides. The neuroendocrine bag cells and exocrine atrial gland cells of Aplysia californica are model peptidergic cells that provide a unique opportunity to examine the processing and differential sorting of a family of egg-laying hormone (ELH)-related polyprotein precursors. Bag cell neurons synthesize and initially cleave the ELH prohormone at a unique tetrabasic site to generate NH. 2-terminal and COOH-terminal intermediates that are sorted to distinct vesicle classes in the trans-Golgi. The processing intermediates subsequently undergo an ordered series of cleavage steps to generate multiple products, including ELH. The atrial gland synthesizes and post-translationally processes large quantities of four ELH-related precursors to liberate multiple products, including ELH-related peptides. Four members of a family of Aplysia subtilisin-related endoproteases that are expressed in bag cell neurons or atrial gland cells have been cloned, and two of these enzymes are capable of correctly cleaving the ELH prohormone. The predicted primary structures of the preproendoproteases suggest that they undergo protcolytic cleavage at the consensus sequence Arg-Xaa-(Lys/Arg)-Arg prior to enzyme activation.

    Original languageEnglish
    Pages (from-to)439-450
    Number of pages12
    JournalNetherlands Journal of Zoology
    Volume44
    Issue number3-4
    DOIs
    StatePublished - 1993

    Fingerprint

    subtilisin
    hormone
    hormones
    oviposition
    egg
    bags
    cells
    peptide
    cleavage
    neurons
    peptides
    enzyme
    enzyme activation
    consensus sequence
    neuropeptides
    vesicle
    sorting
    protein
    enzymes

    Keywords

    • Aplysia californica
    • Atrial gland
    • Bag cells
    • Prohormone convertases

    ASJC Scopus subject areas

    • Animal Science and Zoology

    Cite this

    Molluscan subtilisin-related endoproteases involved in the activation of prohormones and proproteins in the secretory pathway. / Nagle, Gregg T.; Knock, Susan L.; Van heumen, Walter R A; Kurosky, Alexander.

    In: Netherlands Journal of Zoology, Vol. 44, No. 3-4, 1993, p. 439-450.

    Research output: Contribution to journalArticle

    Nagle, Gregg T. ; Knock, Susan L. ; Van heumen, Walter R A ; Kurosky, Alexander. / Molluscan subtilisin-related endoproteases involved in the activation of prohormones and proproteins in the secretory pathway. In: Netherlands Journal of Zoology. 1993 ; Vol. 44, No. 3-4. pp. 439-450.
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