Monitoring neovascularization of intraportal islet grafts by dynamic contrast enhanced magnetic resonance imaging.

Nathaniel Chan, Andre Obenaus, Annie Tan, Naoaki Sakata, John Mace, Ricardo Peverini, Richard Chinnock, Lawrence Sowers, Eba Hathout

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Fifteen thousand youths are diagnosed yearly with type 1 diabetes mellitus. Pancreatic islet transplantation has been shown clinically to provide short-term (~1 year) insulin independence. However, challenges associated with early vascularization of transplanted islet grafts and long-term islet survival remain. We utilized dynamic contrast enhanced magnetic resonance imaging (DCE MRI) to monitor neovascularization of islets transplanted into the right lobe of the liver in a syngeneic mouse model system. The left lobe received no islets and served as a control. DCE data were analyzed for temporal dynamics of contrast (gadolinium) extravasation and the results were fit to a Tofts two-compartment exchange model. We observed maximal right lobe enhancement at seven days post-transplantation. Histological examination up to 28 days was used to confirm imaging results. DCE-derived enhancement strongly correlated with immunohistochemical measures of neovascularization. To our knowledge, these results are the first to demonstrate using a FDA approved contrast agent that DCE MRI can effectively and non-invasively monitor the progression of angiogenesis in intraportal islet grafts.

Original languageEnglish (US)
Pages (from-to)249-255
Number of pages7
JournalIslets
Volume1
Issue number3
StatePublished - Nov 2009
Externally publishedYes

Fingerprint

Magnetic Resonance Imaging
Transplants
Islets of Langerhans Transplantation
Gadolinium
Type 1 Diabetes Mellitus
Contrast Media
Transplantation
Insulin
Liver

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology

Cite this

Chan, N., Obenaus, A., Tan, A., Sakata, N., Mace, J., Peverini, R., ... Hathout, E. (2009). Monitoring neovascularization of intraportal islet grafts by dynamic contrast enhanced magnetic resonance imaging. Islets, 1(3), 249-255.

Monitoring neovascularization of intraportal islet grafts by dynamic contrast enhanced magnetic resonance imaging. / Chan, Nathaniel; Obenaus, Andre; Tan, Annie; Sakata, Naoaki; Mace, John; Peverini, Ricardo; Chinnock, Richard; Sowers, Lawrence; Hathout, Eba.

In: Islets, Vol. 1, No. 3, 11.2009, p. 249-255.

Research output: Contribution to journalArticle

Chan, N, Obenaus, A, Tan, A, Sakata, N, Mace, J, Peverini, R, Chinnock, R, Sowers, L & Hathout, E 2009, 'Monitoring neovascularization of intraportal islet grafts by dynamic contrast enhanced magnetic resonance imaging.', Islets, vol. 1, no. 3, pp. 249-255.
Chan N, Obenaus A, Tan A, Sakata N, Mace J, Peverini R et al. Monitoring neovascularization of intraportal islet grafts by dynamic contrast enhanced magnetic resonance imaging. Islets. 2009 Nov;1(3):249-255.
Chan, Nathaniel ; Obenaus, Andre ; Tan, Annie ; Sakata, Naoaki ; Mace, John ; Peverini, Ricardo ; Chinnock, Richard ; Sowers, Lawrence ; Hathout, Eba. / Monitoring neovascularization of intraportal islet grafts by dynamic contrast enhanced magnetic resonance imaging. In: Islets. 2009 ; Vol. 1, No. 3. pp. 249-255.
@article{8b6a3d115057408c97dd846f43139b7c,
title = "Monitoring neovascularization of intraportal islet grafts by dynamic contrast enhanced magnetic resonance imaging.",
abstract = "Fifteen thousand youths are diagnosed yearly with type 1 diabetes mellitus. Pancreatic islet transplantation has been shown clinically to provide short-term (~1 year) insulin independence. However, challenges associated with early vascularization of transplanted islet grafts and long-term islet survival remain. We utilized dynamic contrast enhanced magnetic resonance imaging (DCE MRI) to monitor neovascularization of islets transplanted into the right lobe of the liver in a syngeneic mouse model system. The left lobe received no islets and served as a control. DCE data were analyzed for temporal dynamics of contrast (gadolinium) extravasation and the results were fit to a Tofts two-compartment exchange model. We observed maximal right lobe enhancement at seven days post-transplantation. Histological examination up to 28 days was used to confirm imaging results. DCE-derived enhancement strongly correlated with immunohistochemical measures of neovascularization. To our knowledge, these results are the first to demonstrate using a FDA approved contrast agent that DCE MRI can effectively and non-invasively monitor the progression of angiogenesis in intraportal islet grafts.",
author = "Nathaniel Chan and Andre Obenaus and Annie Tan and Naoaki Sakata and John Mace and Ricardo Peverini and Richard Chinnock and Lawrence Sowers and Eba Hathout",
year = "2009",
month = "11",
language = "English (US)",
volume = "1",
pages = "249--255",
journal = "Islets",
issn = "1938-2014",
publisher = "Landes Bioscience",
number = "3",

}

TY - JOUR

T1 - Monitoring neovascularization of intraportal islet grafts by dynamic contrast enhanced magnetic resonance imaging.

AU - Chan, Nathaniel

AU - Obenaus, Andre

AU - Tan, Annie

AU - Sakata, Naoaki

AU - Mace, John

AU - Peverini, Ricardo

AU - Chinnock, Richard

AU - Sowers, Lawrence

AU - Hathout, Eba

PY - 2009/11

Y1 - 2009/11

N2 - Fifteen thousand youths are diagnosed yearly with type 1 diabetes mellitus. Pancreatic islet transplantation has been shown clinically to provide short-term (~1 year) insulin independence. However, challenges associated with early vascularization of transplanted islet grafts and long-term islet survival remain. We utilized dynamic contrast enhanced magnetic resonance imaging (DCE MRI) to monitor neovascularization of islets transplanted into the right lobe of the liver in a syngeneic mouse model system. The left lobe received no islets and served as a control. DCE data were analyzed for temporal dynamics of contrast (gadolinium) extravasation and the results were fit to a Tofts two-compartment exchange model. We observed maximal right lobe enhancement at seven days post-transplantation. Histological examination up to 28 days was used to confirm imaging results. DCE-derived enhancement strongly correlated with immunohistochemical measures of neovascularization. To our knowledge, these results are the first to demonstrate using a FDA approved contrast agent that DCE MRI can effectively and non-invasively monitor the progression of angiogenesis in intraportal islet grafts.

AB - Fifteen thousand youths are diagnosed yearly with type 1 diabetes mellitus. Pancreatic islet transplantation has been shown clinically to provide short-term (~1 year) insulin independence. However, challenges associated with early vascularization of transplanted islet grafts and long-term islet survival remain. We utilized dynamic contrast enhanced magnetic resonance imaging (DCE MRI) to monitor neovascularization of islets transplanted into the right lobe of the liver in a syngeneic mouse model system. The left lobe received no islets and served as a control. DCE data were analyzed for temporal dynamics of contrast (gadolinium) extravasation and the results were fit to a Tofts two-compartment exchange model. We observed maximal right lobe enhancement at seven days post-transplantation. Histological examination up to 28 days was used to confirm imaging results. DCE-derived enhancement strongly correlated with immunohistochemical measures of neovascularization. To our knowledge, these results are the first to demonstrate using a FDA approved contrast agent that DCE MRI can effectively and non-invasively monitor the progression of angiogenesis in intraportal islet grafts.

UR - http://www.scopus.com/inward/record.url?scp=77958573690&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77958573690&partnerID=8YFLogxK

M3 - Article

VL - 1

SP - 249

EP - 255

JO - Islets

JF - Islets

SN - 1938-2014

IS - 3

ER -