Monoclonal antibodies to a brain dopamine binding protein

Production, specificity, and immunohistochemistry

L. L. Hsu, K. Moroi, B. D. Lakchaura, T. Chan, C. ‐T Lin, J. ‐Y Wu

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

A dopamine binding protein (DABP) has been purified from the rat brain synaptic membrane to homogeneity by afinity chromatography and gel electrophoresis. The monoclonal antibodies against the DABP were produced by the mouse‐mouse hybridoma technique and characterized for their specificity to dopamine receptors by displacement of dopamine receptor binding. These monoclonal antibodies have been used to localize DABP in rat brain by immunohistochemistry. A specific linear structure of reaction product was seen in both caudate nucleus and cerebral cortex. This finding suggests that the DABP is present in the cerebral cortex and caudate nucleus as a membranous component of the neurons or their processes.

Original languageEnglish (US)
Pages (from-to)417-423
Number of pages7
JournalJournal of Neuroscience Research
Volume17
Issue number4
DOIs
StatePublished - Jan 1 1987

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Dopamine
Carrier Proteins
Immunohistochemistry
Monoclonal Antibodies
Caudate Nucleus
Dopamine Receptors
Brain
Cerebral Cortex
Synaptic Membranes
Hybridomas
Gel Chromatography
Electrophoresis
Neurons

Keywords

  • corpus striatum
  • immunohistochemistry
  • rat brain codex

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

Cite this

Monoclonal antibodies to a brain dopamine binding protein : Production, specificity, and immunohistochemistry. / Hsu, L. L.; Moroi, K.; Lakchaura, B. D.; Chan, T.; Lin, C. ‐T; Wu, J. ‐Y.

In: Journal of Neuroscience Research, Vol. 17, No. 4, 01.01.1987, p. 417-423.

Research output: Contribution to journalArticle

Hsu, L. L. ; Moroi, K. ; Lakchaura, B. D. ; Chan, T. ; Lin, C. ‐T ; Wu, J. ‐Y. / Monoclonal antibodies to a brain dopamine binding protein : Production, specificity, and immunohistochemistry. In: Journal of Neuroscience Research. 1987 ; Vol. 17, No. 4. pp. 417-423.
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