Abstract
Twelve hybridoma clones producing monoclonal antibodies to Afipia felis, a putative causative agent of cat scratch disease, were derived from BALB/c mice immunized with A felis. All 12 monoclonal antibodies were species- specific for A felis and reacted with lipopolysaccharide antigens of A felis. These monoclonal antibodies belong to immunoglobulin (Ig) G1, IgG2a, IgG2b, IgG3, and IgM isotypes. All monoclonal antibodies reacted with both agar- grown A felis and tissue culture-propagated A felis. Formalin fixation did not alter the reactivity of the antigen with the monoclonal antibodies. These monoclonal antibodies could be a useful tool for investigation of the disputed role of A felis in cat scratch disease.
Original language | English (US) |
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Pages (from-to) | 603-606 |
Number of pages | 4 |
Journal | American journal of clinical pathology |
Volume | 101 |
Issue number | 5 |
DOIs | |
State | Published - 1994 |
Externally published | Yes |
Keywords
- Afipia felis
- Cat scratch disease
- Monoclonal antibody
ASJC Scopus subject areas
- Pathology and Forensic Medicine