Monoclonal antibodies to SARS-associated coronavirus (SARS-CoV): Identification of neutralizing and antibodies reactive to S, N, M and e viral proteins

Ralph A. Tripp, Lia M. Haynes, Deborah Moore, Barbara Anderson, Azaibi Tamin, Brian H. Harcourt, Les P. Jones, Mamadi Yilla, Gregory J. Babcock, Thomas Greenough, Donna M. Ambrosino, Rene Alvarez, Justin Callaway, Sheana Cavitt, Kurt Kamrud, Harold Alterson, Jonathan Smith, Jennifer L. Harcourt, Congrong Miao, Raj RazdanJames A. Comer, Pierre E. Rollin, Thomas G. Ksiazek, Anthony Sanchez, Paul A. Rota, William J. Bellini, Larry J. Anderson

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

Monoclonal antibodies (Mabs) against the Urbani strain of the SARS-associated coronavirus (SARS-CoV) were developed and characterized for reactivity to SARS-CoV and SARS-CoV S, N, M, and E proteins using enzyme-linked immunoabsorbent (ELISA), radioimmunoprecipitation, immunofluorescence, Western Blot and microneutralization assays. Twenty-six mAbs were reactive to SARS-CoV by ELISA, and nine were chosen for detailed characterization. Five mAbs reacted against the S protein, two against the M protein, and one each against the N and E proteins. Two of five S protein mAbs neutralized SARS-CoV infection of Vero E6 cells and reacted to an epitope within amino acids 490-510 in the S protein. While two of the three non-neutralizing antibodies recognized at second epitope within amino acids 270-350. The mAbs characterized should prove useful for developing SARS-CoV diagnostic assays and for studying the biology of infection and pathogenesis of disease.

Original languageEnglish (US)
Pages (from-to)21-28
Number of pages8
JournalJournal of Virological Methods
Volume128
Issue number1-2
DOIs
StatePublished - Sep 1 2005

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Keywords

  • Epitope
  • Immunoassay
  • Monoclonal antibody
  • Neutralizing
  • SARS-coronavirus

ASJC Scopus subject areas

  • Virology

Cite this

Tripp, R. A., Haynes, L. M., Moore, D., Anderson, B., Tamin, A., Harcourt, B. H., Jones, L. P., Yilla, M., Babcock, G. J., Greenough, T., Ambrosino, D. M., Alvarez, R., Callaway, J., Cavitt, S., Kamrud, K., Alterson, H., Smith, J., Harcourt, J. L., Miao, C., ... Anderson, L. J. (2005). Monoclonal antibodies to SARS-associated coronavirus (SARS-CoV): Identification of neutralizing and antibodies reactive to S, N, M and e viral proteins. Journal of Virological Methods, 128(1-2), 21-28. https://doi.org/10.1016/j.jviromet.2005.03.021