Monoclonal antibodies to SARS-associated coronavirus (SARS-CoV): Identification of neutralizing and antibodies reactive to S, N, M and e viral proteins

Ralph A. Tripp; Lia M. Haynes; Deborah Moore; Barbara Anderson; Azaibi Tamin; Brian H. Harcourt; Les P. Jones; Mamadi Yilla; Gregory J. Babcock; Thomas Greenough; Donna M. Ambrosino; Rene Alvarez; Justin Callaway; Sheana Cavitt; Kurt Kamrud; Harold Alterson; Jonathan Smith; Jennifer L. Harcourt; Congrong Miao; Raj Razdan; James A. Comer; Pierre E. Rollin; Thomas G. Ksiazek; Anthony Sanchez; Paul A. Rota; William J. Bellini; Larry J. Anderson

doi:10.1016/j.jviromet.2005.03.021

Monoclonal antibodies to SARS-associated coronavirus (SARS-CoV): Identification of neutralizing and antibodies reactive to S, N, M and e viral proteins

Ralph A. Tripp, Lia M. Haynes, Deborah Moore, Barbara Anderson, Azaibi Tamin, Brian H. Harcourt, Les P. Jones, Mamadi Yilla, Gregory J. Babcock, Thomas Greenough, Donna M. Ambrosino, Rene Alvarez, Justin Callaway, Sheana Cavitt, Kurt Kamrud, Harold Alterson, Jonathan Smith, Jennifer L. Harcourt, Congrong Miao, Raj RazdanJames A. Comer, Pierre E. Rollin, Thomas G. Ksiazek, Anthony Sanchez, Paul A. Rota, William J. Bellini, Larry J. Anderson

Research output: Contribution to journal › Article › peer-review

50 Scopus citations

Abstract

Monoclonal antibodies (Mabs) against the Urbani strain of the SARS-associated coronavirus (SARS-CoV) were developed and characterized for reactivity to SARS-CoV and SARS-CoV S, N, M, and E proteins using enzyme-linked immunoabsorbent (ELISA), radioimmunoprecipitation, immunofluorescence, Western Blot and microneutralization assays. Twenty-six mAbs were reactive to SARS-CoV by ELISA, and nine were chosen for detailed characterization. Five mAbs reacted against the S protein, two against the M protein, and one each against the N and E proteins. Two of five S protein mAbs neutralized SARS-CoV infection of Vero E6 cells and reacted to an epitope within amino acids 490-510 in the S protein. While two of the three non-neutralizing antibodies recognized at second epitope within amino acids 270-350. The mAbs characterized should prove useful for developing SARS-CoV diagnostic assays and for studying the biology of infection and pathogenesis of disease.

Original language	English (US)
Pages (from-to)	21-28
Number of pages	8
Journal	Journal of Virological Methods
Volume	128
Issue number	1-2
DOIs	https://doi.org/10.1016/j.jviromet.2005.03.021
State	Published - Sep 2005
Externally published	Yes

Keywords

Epitope
Immunoassay
Monoclonal antibody
Neutralizing
SARS-coronavirus

ASJC Scopus subject areas

Virology

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10.1016/j.jviromet.2005.03.021

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Tripp, R. A., Haynes, L. M., Moore, D., Anderson, B., Tamin, A., Harcourt, B. H., Jones, L. P., Yilla, M., Babcock, G. J., Greenough, T., Ambrosino, D. M., Alvarez, R., Callaway, J., Cavitt, S., Kamrud, K., Alterson, H., Smith, J., Harcourt, J. L., Miao, C., ... Anderson, L. J. (2005). Monoclonal antibodies to SARS-associated coronavirus (SARS-CoV): Identification of neutralizing and antibodies reactive to S, N, M and e viral proteins. Journal of Virological Methods, 128(1-2), 21-28. https://doi.org/10.1016/j.jviromet.2005.03.021

Tripp, RA, Haynes, LM, Moore, D, Anderson, B, Tamin, A, Harcourt, BH, Jones, LP, Yilla, M, Babcock, GJ, Greenough, T, Ambrosino, DM, Alvarez, R, Callaway, J, Cavitt, S, Kamrud, K, Alterson, H, Smith, J, Harcourt, JL, Miao, C, Razdan, R, Comer, JA, Rollin, PE, Ksiazek, TG, Sanchez, A, Rota, PA, Bellini, WJ & Anderson, LJ 2005, 'Monoclonal antibodies to SARS-associated coronavirus (SARS-CoV): Identification of neutralizing and antibodies reactive to S, N, M and e viral proteins', Journal of Virological Methods, vol. 128, no. 1-2, pp. 21-28. https://doi.org/10.1016/j.jviromet.2005.03.021

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title = "Monoclonal antibodies to SARS-associated coronavirus (SARS-CoV): Identification of neutralizing and antibodies reactive to S, N, M and e viral proteins",

abstract = "Monoclonal antibodies (Mabs) against the Urbani strain of the SARS-associated coronavirus (SARS-CoV) were developed and characterized for reactivity to SARS-CoV and SARS-CoV S, N, M, and E proteins using enzyme-linked immunoabsorbent (ELISA), radioimmunoprecipitation, immunofluorescence, Western Blot and microneutralization assays. Twenty-six mAbs were reactive to SARS-CoV by ELISA, and nine were chosen for detailed characterization. Five mAbs reacted against the S protein, two against the M protein, and one each against the N and E proteins. Two of five S protein mAbs neutralized SARS-CoV infection of Vero E6 cells and reacted to an epitope within amino acids 490-510 in the S protein. While two of the three non-neutralizing antibodies recognized at second epitope within amino acids 270-350. The mAbs characterized should prove useful for developing SARS-CoV diagnostic assays and for studying the biology of infection and pathogenesis of disease.",

keywords = "Epitope, Immunoassay, Monoclonal antibody, Neutralizing, SARS-coronavirus",

author = "Tripp, {Ralph A.} and Haynes, {Lia M.} and Deborah Moore and Barbara Anderson and Azaibi Tamin and Harcourt, {Brian H.} and Jones, {Les P.} and Mamadi Yilla and Babcock, {Gregory J.} and Thomas Greenough and Ambrosino, {Donna M.} and Rene Alvarez and Justin Callaway and Sheana Cavitt and Kurt Kamrud and Harold Alterson and Jonathan Smith and Harcourt, {Jennifer L.} and Congrong Miao and Raj Razdan and Comer, {James A.} and Rollin, {Pierre E.} and Ksiazek, {Thomas G.} and Anthony Sanchez and Rota, {Paul A.} and Bellini, {William J.} and Anderson, {Larry J.}",

year = "2005",

month = sep,

doi = "10.1016/j.jviromet.2005.03.021",

language = "English (US)",

volume = "128",

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journal = "Journal of Virological Methods",

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T2 - Identification of neutralizing and antibodies reactive to S, N, M and e viral proteins

AU - Tripp, Ralph A.

AU - Haynes, Lia M.

AU - Moore, Deborah

AU - Anderson, Barbara

AU - Tamin, Azaibi

AU - Harcourt, Brian H.

AU - Jones, Les P.

AU - Yilla, Mamadi

AU - Babcock, Gregory J.

AU - Greenough, Thomas

AU - Ambrosino, Donna M.

AU - Alvarez, Rene

AU - Callaway, Justin

AU - Cavitt, Sheana

AU - Kamrud, Kurt

AU - Alterson, Harold

AU - Smith, Jonathan

AU - Harcourt, Jennifer L.

AU - Miao, Congrong

AU - Razdan, Raj

AU - Comer, James A.

AU - Rollin, Pierre E.

AU - Ksiazek, Thomas G.

AU - Sanchez, Anthony

AU - Rota, Paul A.

AU - Bellini, William J.

AU - Anderson, Larry J.

PY - 2005/9

Y1 - 2005/9

N2 - Monoclonal antibodies (Mabs) against the Urbani strain of the SARS-associated coronavirus (SARS-CoV) were developed and characterized for reactivity to SARS-CoV and SARS-CoV S, N, M, and E proteins using enzyme-linked immunoabsorbent (ELISA), radioimmunoprecipitation, immunofluorescence, Western Blot and microneutralization assays. Twenty-six mAbs were reactive to SARS-CoV by ELISA, and nine were chosen for detailed characterization. Five mAbs reacted against the S protein, two against the M protein, and one each against the N and E proteins. Two of five S protein mAbs neutralized SARS-CoV infection of Vero E6 cells and reacted to an epitope within amino acids 490-510 in the S protein. While two of the three non-neutralizing antibodies recognized at second epitope within amino acids 270-350. The mAbs characterized should prove useful for developing SARS-CoV diagnostic assays and for studying the biology of infection and pathogenesis of disease.

AB - Monoclonal antibodies (Mabs) against the Urbani strain of the SARS-associated coronavirus (SARS-CoV) were developed and characterized for reactivity to SARS-CoV and SARS-CoV S, N, M, and E proteins using enzyme-linked immunoabsorbent (ELISA), radioimmunoprecipitation, immunofluorescence, Western Blot and microneutralization assays. Twenty-six mAbs were reactive to SARS-CoV by ELISA, and nine were chosen for detailed characterization. Five mAbs reacted against the S protein, two against the M protein, and one each against the N and E proteins. Two of five S protein mAbs neutralized SARS-CoV infection of Vero E6 cells and reacted to an epitope within amino acids 490-510 in the S protein. While two of the three non-neutralizing antibodies recognized at second epitope within amino acids 270-350. The mAbs characterized should prove useful for developing SARS-CoV diagnostic assays and for studying the biology of infection and pathogenesis of disease.

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KW - Immunoassay

KW - Monoclonal antibody

KW - Neutralizing

KW - SARS-coronavirus

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ER -

Monoclonal antibodies to SARS-associated coronavirus (SARS-CoV): Identification of neutralizing and antibodies reactive to S, N, M and e viral proteins

Abstract

Keywords

ASJC Scopus subject areas

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