Monocyte chemoattractant protein-1 production by intestinal myofibroblasts in response to staphylococcal enterotoxin A

Relevance to staphylococcal enterotoxigenic disease

Iryna Pinchuk, Ellen J. Beswick, Jamal I. Saada, Giovanni Suarez, John Winston, Randy C. Mifflin, John F. Di Mari, Don W. Powell, Victor Reyes

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Food poisoning due to staphylococcal enterotoxins A and B (SEA and SEB) affects hundreds of thousands of people annually. SEA and SEB induce massive intestinal cytokine production, which is believed to be the key factor in staphylococcal enterotoxin enteropathy. MHC class II molecules are the major receptors for staphylococcal enterotoxins. We recently demonstrated that normal human subepithelial intestinal myofibroblasts (IMFs) express MHC class II molecules. We hypothesized that IMFs are among the first cells to respond to staphylococcal enterotoxins and contribute to the cytokine production associated with staphylococcal enterotoxin pathogenesis. We demonstrated here that primary cultured IMFs bind staphylococcal enterotoxins in a MHC class II-dependent fashion in vitro. We also demonstrated that staphylococcal enterotoxins can cross a CaCo-2 epithelial monolayer in coculture with IMFs and bind to the MHC class II on IMFs. IMFs responded to SEA, but not SEB, exposure with 3- to 20-fold increases in the production of proinflammatory chemokines (MCP-1, IL-8), cytokines (IL-6), and growth factors (GM-CSF and G-CSF). The SEA induction of the proinflammatory mediators by IMFs resulted from the efficient cross-linking of MHC class II molecules because cross-linking of class II MHC by biotinylated anti-HLA-DR Abs induced similar cytokine patterns. The studies presented here show that MCP-1 is central to the production of other cytokines elicited by SEA in IMFs because its neutralization with specific Abs prevented the expression of IL-6 and IL-8 by IMFs. Thus, MCP-1 may play a leading role in initiation of inflammatory injury associated with staphylococcal enterotoxigenic disease.

Original languageEnglish (US)
Pages (from-to)8097-8106
Number of pages10
JournalJournal of Immunology
Volume178
Issue number12
StatePublished - Jun 15 2007

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Myofibroblasts
Chemokine CCL2
Enterotoxins
Cytokines
Interleukin-8
Interleukin-6
Staphylococcal enterotoxin A
Foodborne Diseases
HLA-DR Antigens
Granulocyte Colony-Stimulating Factor
Granulocyte-Macrophage Colony-Stimulating Factor
Coculture Techniques
Chemokines
Intercellular Signaling Peptides and Proteins
Wounds and Injuries

ASJC Scopus subject areas

  • Immunology

Cite this

Monocyte chemoattractant protein-1 production by intestinal myofibroblasts in response to staphylococcal enterotoxin A : Relevance to staphylococcal enterotoxigenic disease. / Pinchuk, Iryna; Beswick, Ellen J.; Saada, Jamal I.; Suarez, Giovanni; Winston, John; Mifflin, Randy C.; Di Mari, John F.; Powell, Don W.; Reyes, Victor.

In: Journal of Immunology, Vol. 178, No. 12, 15.06.2007, p. 8097-8106.

Research output: Contribution to journalArticle

Pinchuk, Iryna ; Beswick, Ellen J. ; Saada, Jamal I. ; Suarez, Giovanni ; Winston, John ; Mifflin, Randy C. ; Di Mari, John F. ; Powell, Don W. ; Reyes, Victor. / Monocyte chemoattractant protein-1 production by intestinal myofibroblasts in response to staphylococcal enterotoxin A : Relevance to staphylococcal enterotoxigenic disease. In: Journal of Immunology. 2007 ; Vol. 178, No. 12. pp. 8097-8106.
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