Monounsaturated fatty acids are substrates for aldehyde generation in tellurite-exposed Escherichia coli

Gonzalo A. Pradenas, Waldo A. Díaz-Vásquez, José M. Pérez-Donoso, Claudio C. Vásquez

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Reactive oxygen species (ROS) damage macromolecules and cellular components in nearly all kinds of cells and often generate toxic intracellular byproducts. In this work, aldehyde generation derived from the Escherichia coli membrane oxidation as well as membrane fatty acid profiles, protein oxidation, and bacterial resistance to oxidative stress elicitors was evaluated. Studies included wild-type cells as well as cells exhibiting a modulated monounsaturated fatty acid (MUFA) ratio. The hydroxyaldehyde 4-hydroxy 2-nonenal was found to be most likely produced by E. coli, whose levels are dependent upon exposure to oxidative stress elicitors. Aldehyde amounts and markers of oxidative damage decreased upon exposure to E. coli containing low MUFA ratios, which was paralleled by a concomitant increase in resistance to ROS-generating compounds. MUFAs ratio, lipid peroxidation, and aldehyde generation were found to be directly related; that is, the lower the MUFAs ratio, the lower the peroxide and aldehyde generation levels. These results provide additional evidence about MUFAs being targets for membrane lipid oxidation and their relevance in aldehyde generation.

Original languageEnglish (US)
Article number563756
JournalBioMed Research International
Volume2013
DOIs
StatePublished - 2013
Externally publishedYes

Fingerprint

Monounsaturated Fatty Acids
Aldehydes
Escherichia coli
Substrates
Oxidative stress
Oxidation
Reactive Oxygen Species
Oxidative Stress
Membranes
Bacterial Proteins
Poisons
Peroxides
Membrane Lipids
Macromolecules
Lipid Peroxidation
Byproducts
Fatty Acids
tellurous acid
Lipids
Proteins

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Medicine(all)

Cite this

Monounsaturated fatty acids are substrates for aldehyde generation in tellurite-exposed Escherichia coli. / Pradenas, Gonzalo A.; Díaz-Vásquez, Waldo A.; Pérez-Donoso, José M.; Vásquez, Claudio C.

In: BioMed Research International, Vol. 2013, 563756, 2013.

Research output: Contribution to journalArticle

Pradenas, Gonzalo A. ; Díaz-Vásquez, Waldo A. ; Pérez-Donoso, José M. ; Vásquez, Claudio C. / Monounsaturated fatty acids are substrates for aldehyde generation in tellurite-exposed Escherichia coli. In: BioMed Research International. 2013 ; Vol. 2013.
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