Morbillivirus and henipavirus attachment protein cytoplasmic domains differently affect protein expression, fusion support and particle assembly

Bevan Sawatsky, Dennis Bente, Markus Czub, Veronika von Messling

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The amino-terminal cytoplasmic domains of paramyxovirus attachment glycoproteins include trafficking signals that influence protein processing and cell surface expression. To characterize the role of the cytoplasmic domain in protein expression, fusion support and particle assembly in more detail, we constructed chimeric Nipah virus (NiV) glycoprotein (G) and canine distemper virus (CDV) haemagglutinin (H) proteins carrying the respective heterologous cytoplasmic domain, as well as a series of mutants with progressive deletions in this domain. CDV H retained fusion function and was normally expressed on the cell surface with a heterologous cytoplasmic domain, while the expression and fusion support of NiV G was dramatically decreased when its cytoplasmic domain was replaced with that of CDV H. The cell surface expression and fusion support functions of CDV H were relatively insensitive to cytoplasmic domain deletions, while short deletions in the corresponding region of NiV G dramatically decreased both. In addition, the first 10 residues of the CDV H cytoplasmic domain strongly influence its incorporation into virus-like particles formed by the CDV matrix (M) protein, while the co-expression of NiV M with NiV G had no significant effect on incorporation of G into particles. The cytoplasmic domains of both the CDV H and NiV G proteins thus contribute differently to the virus life cycle.

Original languageEnglish (US)
Article number000415
Pages (from-to)1066-1076
Number of pages11
JournalJournal of General Virology
Volume97
Issue number5
DOIs
StatePublished - May 1 2016

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Henipavirus
Morbillivirus
Canine Distemper Virus
Nipah Virus
Proteins
Glycoproteins
Hemagglutinins
Protein Domains
Life Cycle Stages
GTP-Binding Proteins
Virion
Membrane Proteins
Viruses

ASJC Scopus subject areas

  • Virology

Cite this

Morbillivirus and henipavirus attachment protein cytoplasmic domains differently affect protein expression, fusion support and particle assembly. / Sawatsky, Bevan; Bente, Dennis; Czub, Markus; von Messling, Veronika.

In: Journal of General Virology, Vol. 97, No. 5, 000415, 01.05.2016, p. 1066-1076.

Research output: Contribution to journalArticle

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