More efficient peptide binding to MHC class II molecules during cathepsin B digestion of Ii than after Ii release

Masanori Daibata, Minzhen Xu, Robert E. Humphreys, Victor Reyes

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The binding of a T cell-presented peptide to MHC class II α,β chains occurs as a concurrent process with the release of the associated invariant chain (Ii) by cathepsin B. Ii was digested by cathepsin B from solubilized, MHC class II α,β,Ii complexes in the presence of N-hydroxysuccinimidyl-4-azidobenzoate-conjugated, 125I-labeled, influenza virus matrix (18-29) peptide. The peptide was crosslinked where it became bound. This HLA-DR1-restricted peptide bound about three times more efficiently to class II α,β chains of DR 1- positive B cells when present during cathepsin B digestion of Ii than when added afterward, also at pH 5.0. Binding was competed by similarly DR-restricted peptides. Cathepsin D cleaved Ii but did not enhance peptide binding. However, a trace level of cathepsin D, added to the assay for peptide binding in the presence of cathepsin B, further enhanced peptide binding about three times. These experiments support an hypothesis for the staged release of Ii fragments by cathepsin D and cathepsin B, catalyzing at one point the insertion of a peptide into the antigen binding site formed by class II α and β chains.

Original languageEnglish (US)
Pages (from-to)255-260
Number of pages6
JournalMolecular Immunology
Volume31
Issue number4
DOIs
StatePublished - 1994

Fingerprint

Cathepsin B
Digestion
Peptides
Cathepsin D
HLA-DR1 Antigen
MHC binding peptide
Orthomyxoviridae
B-Lymphocytes
Binding Sites
T-Lymphocytes
Antigens

ASJC Scopus subject areas

  • Molecular Biology
  • Immunology

Cite this

More efficient peptide binding to MHC class II molecules during cathepsin B digestion of Ii than after Ii release. / Daibata, Masanori; Xu, Minzhen; Humphreys, Robert E.; Reyes, Victor.

In: Molecular Immunology, Vol. 31, No. 4, 1994, p. 255-260.

Research output: Contribution to journalArticle

Daibata, Masanori ; Xu, Minzhen ; Humphreys, Robert E. ; Reyes, Victor. / More efficient peptide binding to MHC class II molecules during cathepsin B digestion of Ii than after Ii release. In: Molecular Immunology. 1994 ; Vol. 31, No. 4. pp. 255-260.
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