Morphogenesis of the T4 tail and tail fibers

Petr Leiman, Fumio Arisaka, Mark J. Van Raaij, Victor A. Kostyuchenko, Anastasia A. Aksyuk, Shuji Kanamaru, Michael G. Rossmann

Research output: Contribution to journalReview article

130 Citations (Scopus)

Abstract

Remarkable progress has been made during the past ten years in elucidating the structure of the bacteriophage T4 tail by a combination of three-dimensional image reconstruction from electron micrographs and X-ray crystallography of the components. Partial and complete structures of nine out of twenty tail structural proteins have been determined by X-ray crystallography and have been fitted into the 3D-reconstituted structure of the "extended" tail. The 3D structure of the "contracted" tail was also determined and interpreted in terms of component proteins. Given the pseudo-atomic tail structures both before and after contraction, it is now possible to understand the gross conformational change of the baseplate in terms of the change in the relative positions of the subunit proteins. These studies have explained how the conformational change of the baseplate and contraction of the tail are related to the tail's host cell recognition and membrane penetration function. On the other hand, the baseplate assembly process has been recently reexamined in detail in a precise system involving recombinant proteins (unlike the earlier studies with phage mutants). These experiments showed that the sequential association of the subunits of the baseplate wedge is based on the induced-fit upon association of each subunit. It was also found that, upon association of gp53 (gene product 53), the penultimate subunit of the wedge, six of the wedge intermediates spontaneously associate to form a baseplate-like structure in the absence of the central hub. Structure determination of the rest of the subunits and intermediate complexes and the assembly of the hub still require further study.

Original languageEnglish (US)
Article number355
JournalVirology Journal
Volume7
DOIs
StatePublished - 2010
Externally publishedYes

Fingerprint

X Ray Crystallography
Morphogenesis
Bacteriophage T4
Computer-Assisted Image Processing
Three-Dimensional Imaging
Protein Subunits
Recombinant Proteins
Bacteriophages
Proteins
Cell Membrane
Electrons
Genes

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Leiman, P., Arisaka, F., Van Raaij, M. J., Kostyuchenko, V. A., Aksyuk, A. A., Kanamaru, S., & Rossmann, M. G. (2010). Morphogenesis of the T4 tail and tail fibers. Virology Journal, 7, [355]. https://doi.org/10.1186/1743-422X-7-355

Morphogenesis of the T4 tail and tail fibers. / Leiman, Petr; Arisaka, Fumio; Van Raaij, Mark J.; Kostyuchenko, Victor A.; Aksyuk, Anastasia A.; Kanamaru, Shuji; Rossmann, Michael G.

In: Virology Journal, Vol. 7, 355, 2010.

Research output: Contribution to journalReview article

Leiman, P, Arisaka, F, Van Raaij, MJ, Kostyuchenko, VA, Aksyuk, AA, Kanamaru, S & Rossmann, MG 2010, 'Morphogenesis of the T4 tail and tail fibers', Virology Journal, vol. 7, 355. https://doi.org/10.1186/1743-422X-7-355
Leiman P, Arisaka F, Van Raaij MJ, Kostyuchenko VA, Aksyuk AA, Kanamaru S et al. Morphogenesis of the T4 tail and tail fibers. Virology Journal. 2010;7. 355. https://doi.org/10.1186/1743-422X-7-355
Leiman, Petr ; Arisaka, Fumio ; Van Raaij, Mark J. ; Kostyuchenko, Victor A. ; Aksyuk, Anastasia A. ; Kanamaru, Shuji ; Rossmann, Michael G. / Morphogenesis of the T4 tail and tail fibers. In: Virology Journal. 2010 ; Vol. 7.
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