Características morfológicas y electrofisiológicas de las neuronas del ganglio vestibular en cultivo

Translated title of the contribution: Morphologic and electrophysiologic characteristics of vestibular ganglion neurons in culture

Enrique Soto, Agenor Limon-Ruiz, Aída Ortega, Rosario Vega

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Vestibular afferent neurons have been classified on the basis of their spontaneous activity as regular and irregular; this has been attributed to their synaptic input, but it remains to be defined the participation of some intrinsical properties of the afferent neurons in the determination of their discharge pattern. In this work, we have developed-tissue cultures of the rat vestibular ganglia. Isolated cells were plated using poly-D-lysine or collagen as substrates and L-15 or Neurobasal® as culture media. After 48 hrs cells in the four experimental conditions give forth neurites of variable longitude. By using antibodies against the neurofilaments 160 kDa the cell structure was studied. Monopolar (30.6%), bipolar (63.9%) and multipolar (5.5%) cells were found. By using the voltage and current clamp procedures the voltage dependence and kinetics of the tetrodotoxin sensitive Na+ current was fully characterized. Cultured cells were shown to generate action potentials under electrical stimulation, and they were capable of repetitive spike discharge under the influence of 4-aminopyridine. These results demonstrate that tissue cultures constitute an excellent system to study the intrinsical properties of vestibular afferent neurons.

Original languageSpanish
Pages (from-to)1-13
Number of pages13
JournalGaceta Medica de Mexico
Volume138
Issue number1
StatePublished - Mar 14 2002
Externally publishedYes

Fingerprint

Afferent Neurons
Ganglia
Neurons
4-Aminopyridine
Intermediate Filaments
Tetrodotoxin
Neurites
Electric Stimulation
Action Potentials
Lysine
Culture Media
Cultured Cells
Collagen
Antibodies

Keywords

  • Inner ear
  • Primary culture
  • Sensory coding
  • Sodium current
  • Vestibular afferent neurons
  • Voltage clamp

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Características morfológicas y electrofisiológicas de las neuronas del ganglio vestibular en cultivo. / Soto, Enrique; Limon-Ruiz, Agenor; Ortega, Aída; Vega, Rosario.

In: Gaceta Medica de Mexico, Vol. 138, No. 1, 14.03.2002, p. 1-13.

Research output: Contribution to journalArticle

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