Morphological and cytochemical study of Chlamydia with EDTA regressive technique and Gautier staining in ultrathin frozen sections of infected cell cultures

a comparison with embedded material

Vsevolod Popov, F. Eb, J. F. Lefebvre, J. Orfila, A. Viron

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The cryo-ultramicrotomy technique was applied to study the ultrastructure of Chlamydia using two strains: one of C. psittaci and one of C. trachomatis. It clearly appeared that in both strains reticulate bodies show a high degree of plasticity, in contrast with the rigid spherical appearance of elementary bodies. Ultrastructural cytochemical study shows DNA fibrils dispersed throughout the cytoplasm in reticulate bodies whereas DNA is condensed in a nucleoid in elementary and intermediate bodies. The EDTA regressive technique reveals ribonucleoproteins in reticulate and elementary bodies of both studied strains.

Original languageEnglish (US)
Pages (from-to)313-337
Number of pages25
JournalAnnales de Microbiologie
Volume129 B
Issue number3
StatePublished - 1978
Externally publishedYes

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Chlamydia
Frozen Sections
Edetic Acid
Cell Culture Techniques
Microtomy
Staining and Labeling
Ribonucleoproteins
DNA
Cytoplasm

ASJC Scopus subject areas

  • Medicine(all)

Cite this

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abstract = "The cryo-ultramicrotomy technique was applied to study the ultrastructure of Chlamydia using two strains: one of C. psittaci and one of C. trachomatis. It clearly appeared that in both strains reticulate bodies show a high degree of plasticity, in contrast with the rigid spherical appearance of elementary bodies. Ultrastructural cytochemical study shows DNA fibrils dispersed throughout the cytoplasm in reticulate bodies whereas DNA is condensed in a nucleoid in elementary and intermediate bodies. The EDTA regressive technique reveals ribonucleoproteins in reticulate and elementary bodies of both studied strains.",
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T1 - Morphological and cytochemical study of Chlamydia with EDTA regressive technique and Gautier staining in ultrathin frozen sections of infected cell cultures

T2 - a comparison with embedded material

AU - Popov, Vsevolod

AU - Eb, F.

AU - Lefebvre, J. F.

AU - Orfila, J.

AU - Viron, A.

PY - 1978

Y1 - 1978

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AB - The cryo-ultramicrotomy technique was applied to study the ultrastructure of Chlamydia using two strains: one of C. psittaci and one of C. trachomatis. It clearly appeared that in both strains reticulate bodies show a high degree of plasticity, in contrast with the rigid spherical appearance of elementary bodies. Ultrastructural cytochemical study shows DNA fibrils dispersed throughout the cytoplasm in reticulate bodies whereas DNA is condensed in a nucleoid in elementary and intermediate bodies. The EDTA regressive technique reveals ribonucleoproteins in reticulate and elementary bodies of both studied strains.

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