Morphological and physiological plasticity of spinal lamina II GABA neurons is induced by sciatic nerve chronic constriction injury in mice

Hongmei Zhang, Yan Li, Qing Yang, Xian Guo Liu, Patrick M. Dougherty

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Mice with transgenic insertion of code for enhanced green fluorescent protein (EGFP) at the locus for glutamic acid decarboxylase 67 (GAD67), one of two key enzymes for the synthesis of γ-aminobutyric acid (GABA) were used to test whether the morphological properties of these neurons show plasticity with nerve injury. Physiological properties and the delivery of intracellular label to EGFP-expressing lamina II neurons was done using whole-cell patch-clamp in spinal cord slices from sham and chronic constriction injury (CCI) mice. As well, whole cell recordings were made of non-EGFP labeled cells to ascertain changes in overall inhibitory signaling following CCI. The EGFP labeled neurons in both sham and CCI mice exhibited islet, central and vertical cell morphological profiles but no radial cell profiles were observed. The length of cell dendrites was found to be significantly shorter in CCI mice for all cell profile types. The longest neurites averaged 155.96 ± 18.29 µm in CCI mice compared to 334.93 ± 29.48 µm in sham control mice. No change was observed in either passive or evoked membrane properties of EGFP-expressing neurons in CCI versus sham mice. Meanwhile, the frequency of miniature inhibitory post-synaptic currents of non-EGFP expressing spinal lamina II neurons was significantly reduced. These results suggest that reduced inhibitory output from GABA neurons occurs with nerve injury in part due to altered cell morphology.

Original languageEnglish (US)
Article number143
JournalFrontiers in Cellular Neuroscience
Volume12
DOIs
StatePublished - May 24 2018
Externally publishedYes

Fingerprint

Substantia Gelatinosa
GABAergic Neurons
Sciatic Nerve
Constriction
Wounds and Injuries
Neurons
Green Fluorescent Proteins
Aminobutyrates
Glutamate Decarboxylase
Patch-Clamp Techniques
Neurites
Dendrites
gamma-Aminobutyric Acid
Transgenic Mice
Spinal Cord

Keywords

  • Disinhibition
  • IPSC
  • Neuropathic pain
  • Patch clamp

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

Cite this

Morphological and physiological plasticity of spinal lamina II GABA neurons is induced by sciatic nerve chronic constriction injury in mice. / Zhang, Hongmei; Li, Yan; Yang, Qing; Liu, Xian Guo; Dougherty, Patrick M.

In: Frontiers in Cellular Neuroscience, Vol. 12, 143, 24.05.2018.

Research output: Contribution to journalArticle

@article{286a5bc9acd94ffea4d1e01f81cb903f,
title = "Morphological and physiological plasticity of spinal lamina II GABA neurons is induced by sciatic nerve chronic constriction injury in mice",
abstract = "Mice with transgenic insertion of code for enhanced green fluorescent protein (EGFP) at the locus for glutamic acid decarboxylase 67 (GAD67), one of two key enzymes for the synthesis of γ-aminobutyric acid (GABA) were used to test whether the morphological properties of these neurons show plasticity with nerve injury. Physiological properties and the delivery of intracellular label to EGFP-expressing lamina II neurons was done using whole-cell patch-clamp in spinal cord slices from sham and chronic constriction injury (CCI) mice. As well, whole cell recordings were made of non-EGFP labeled cells to ascertain changes in overall inhibitory signaling following CCI. The EGFP labeled neurons in both sham and CCI mice exhibited islet, central and vertical cell morphological profiles but no radial cell profiles were observed. The length of cell dendrites was found to be significantly shorter in CCI mice for all cell profile types. The longest neurites averaged 155.96 ± 18.29 µm in CCI mice compared to 334.93 ± 29.48 µm in sham control mice. No change was observed in either passive or evoked membrane properties of EGFP-expressing neurons in CCI versus sham mice. Meanwhile, the frequency of miniature inhibitory post-synaptic currents of non-EGFP expressing spinal lamina II neurons was significantly reduced. These results suggest that reduced inhibitory output from GABA neurons occurs with nerve injury in part due to altered cell morphology.",
keywords = "Disinhibition, IPSC, Neuropathic pain, Patch clamp",
author = "Hongmei Zhang and Yan Li and Qing Yang and Liu, {Xian Guo} and Dougherty, {Patrick M.}",
year = "2018",
month = "5",
day = "24",
doi = "10.3389/fncel.2018.00143",
language = "English (US)",
volume = "12",
journal = "Frontiers in Cellular Neuroscience",
issn = "1662-5102",
publisher = "Frontiers Research Foundation",

}

TY - JOUR

T1 - Morphological and physiological plasticity of spinal lamina II GABA neurons is induced by sciatic nerve chronic constriction injury in mice

AU - Zhang, Hongmei

AU - Li, Yan

AU - Yang, Qing

AU - Liu, Xian Guo

AU - Dougherty, Patrick M.

PY - 2018/5/24

Y1 - 2018/5/24

N2 - Mice with transgenic insertion of code for enhanced green fluorescent protein (EGFP) at the locus for glutamic acid decarboxylase 67 (GAD67), one of two key enzymes for the synthesis of γ-aminobutyric acid (GABA) were used to test whether the morphological properties of these neurons show plasticity with nerve injury. Physiological properties and the delivery of intracellular label to EGFP-expressing lamina II neurons was done using whole-cell patch-clamp in spinal cord slices from sham and chronic constriction injury (CCI) mice. As well, whole cell recordings were made of non-EGFP labeled cells to ascertain changes in overall inhibitory signaling following CCI. The EGFP labeled neurons in both sham and CCI mice exhibited islet, central and vertical cell morphological profiles but no radial cell profiles were observed. The length of cell dendrites was found to be significantly shorter in CCI mice for all cell profile types. The longest neurites averaged 155.96 ± 18.29 µm in CCI mice compared to 334.93 ± 29.48 µm in sham control mice. No change was observed in either passive or evoked membrane properties of EGFP-expressing neurons in CCI versus sham mice. Meanwhile, the frequency of miniature inhibitory post-synaptic currents of non-EGFP expressing spinal lamina II neurons was significantly reduced. These results suggest that reduced inhibitory output from GABA neurons occurs with nerve injury in part due to altered cell morphology.

AB - Mice with transgenic insertion of code for enhanced green fluorescent protein (EGFP) at the locus for glutamic acid decarboxylase 67 (GAD67), one of two key enzymes for the synthesis of γ-aminobutyric acid (GABA) were used to test whether the morphological properties of these neurons show plasticity with nerve injury. Physiological properties and the delivery of intracellular label to EGFP-expressing lamina II neurons was done using whole-cell patch-clamp in spinal cord slices from sham and chronic constriction injury (CCI) mice. As well, whole cell recordings were made of non-EGFP labeled cells to ascertain changes in overall inhibitory signaling following CCI. The EGFP labeled neurons in both sham and CCI mice exhibited islet, central and vertical cell morphological profiles but no radial cell profiles were observed. The length of cell dendrites was found to be significantly shorter in CCI mice for all cell profile types. The longest neurites averaged 155.96 ± 18.29 µm in CCI mice compared to 334.93 ± 29.48 µm in sham control mice. No change was observed in either passive or evoked membrane properties of EGFP-expressing neurons in CCI versus sham mice. Meanwhile, the frequency of miniature inhibitory post-synaptic currents of non-EGFP expressing spinal lamina II neurons was significantly reduced. These results suggest that reduced inhibitory output from GABA neurons occurs with nerve injury in part due to altered cell morphology.

KW - Disinhibition

KW - IPSC

KW - Neuropathic pain

KW - Patch clamp

UR - http://www.scopus.com/inward/record.url?scp=85048962841&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85048962841&partnerID=8YFLogxK

U2 - 10.3389/fncel.2018.00143

DO - 10.3389/fncel.2018.00143

M3 - Article

AN - SCOPUS:85048962841

VL - 12

JO - Frontiers in Cellular Neuroscience

JF - Frontiers in Cellular Neuroscience

SN - 1662-5102

M1 - 143

ER -