Morphological changes in subcutaneous white adipose tissue after severe burn injury

Manish Kumar Saraf, David Herndon, Craig Porter, Tracy Toliver-Kinsky, Ravi Radhakrishnan, Tony Chao, Maria Chondronikola, Labros S. Sidossis

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Severe burn injury produces a plethora of metabolic abnormalities which contribute to the prolonged morbidity of burn survivors. The authors have recently demonstrated trans-differentiation of white adipose tissue (WAT) after burn trauma, toward a more thermogenic phenotype. However, the impact of burn injury on subcutaneous WAT (sWAT) morphology in humans is unknown. Here, the authors studied the effect of severe burn injury on the architecture of sWAT. sWAT was collected from 11 severely burned children (11 ± 3 years; 55 ± 16% total BSA burned) and 12 nonburned healthy children (9 ± 3 years). Histology, electron microscopy, immunohistochemistry, and immunofluorescence were performed on fixed adipose tissue sections. sWAT cytokine and collagen concentrations were measured by multiplex assay and sirius/fast green staining method, respectively. sWAT histology demonstrated multiple fat droplets, significantly (P

Original languageEnglish (US)
Pages (from-to)e96-e103
JournalJournal of Burn Care and Research
Volume37
Issue number2
DOIs
StatePublished - 2016

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White Adipose Tissue
Subcutaneous Fat
Wounds and Injuries
Histology
Fluorescent Antibody Technique
Survivors
Adipose Tissue
Electron Microscopy
Collagen
Immunohistochemistry
Fats
Staining and Labeling
Cytokines
Morbidity
Phenotype

ASJC Scopus subject areas

  • Emergency Medicine
  • Rehabilitation
  • Surgery

Cite this

Morphological changes in subcutaneous white adipose tissue after severe burn injury. / Saraf, Manish Kumar; Herndon, David; Porter, Craig; Toliver-Kinsky, Tracy; Radhakrishnan, Ravi; Chao, Tony; Chondronikola, Maria; Sidossis, Labros S.

In: Journal of Burn Care and Research, Vol. 37, No. 2, 2016, p. e96-e103.

Research output: Contribution to journalArticle

Saraf, Manish Kumar ; Herndon, David ; Porter, Craig ; Toliver-Kinsky, Tracy ; Radhakrishnan, Ravi ; Chao, Tony ; Chondronikola, Maria ; Sidossis, Labros S. / Morphological changes in subcutaneous white adipose tissue after severe burn injury. In: Journal of Burn Care and Research. 2016 ; Vol. 37, No. 2. pp. e96-e103.
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