Morphological effects and metabolism of the molting hormone in Aedes aegypti cultured cells

Ephraim Cohen, Naomi Lanir, Ella Englander

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Growth of Aedes aegypti cultured cells was arrested by α- and β-ecdysone at concentrations of 0.01 to 10.0 μg/ml. The inhibitory effect was accompanied by increased cell volume. Prolonged exposure of at least 24 hr to the molting hormones was necessary to induce the above effects. 3H-α-ecdysone was incorporated into the mosquito cultured cells, and 2.1% of the total label added could be detected in thoroughly washed cells. Thin-layer chromatography of the cellular butanolic extract revealed one apolar peak only and no traces of the original labeled α-ecdysone or its immediate hydroxylation product, e.g. β-ecdysone. It is suggested that the hormone was rapidly converted to a metabolite which did not correspond with the apolar 3-α-dehydro-ecdysone, as was initially speculated.

Original languageEnglish (US)
Pages (from-to)433-439
Number of pages7
JournalInsect Biochemistry
Volume6
Issue number4
DOIs
StatePublished - Jan 1 1976
Externally publishedYes

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Ecdysone
ecdysone
Aedes
ecdysteroids
Aedes aegypti
Metabolism
cultured cells
Cultured Cells
Cells
metabolism
hydroxylation
Thin layer chromatography
Hydroxylation
thin layer chromatography
Thin Layer Chromatography
Metabolites
Culicidae
Cell Size
hormones
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Morphological effects and metabolism of the molting hormone in Aedes aegypti cultured cells. / Cohen, Ephraim; Lanir, Naomi; Englander, Ella.

In: Insect Biochemistry, Vol. 6, No. 4, 01.01.1976, p. 433-439.

Research output: Contribution to journalArticle

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