Morphological effects and metabolism of the molting hormone in Aedes aegypti cultured cells

Ephraim Cohen, Naomi Lanir, Ela Englander

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Growth of Aedes aegypti cultured cells was arrested by α- and β-ecdysone at concentrations of 0.01 to 10.0 μg/ml. The inhibitory effect was accompanied by increased cell volume. Prolonged exposure of at least 24 hr to the molting hormones was necessary to induce the above effects. 3H-α-ecdysone was incorporated into the mosquito cultured cells, and 2.1% of the total label added could be detected in thoroughly washed cells. Thin-layer chromatography of the cellular butanolic extract revealed one apolar peak only and no traces of the original labeled α-ecdysone or its immediate hydroxylation product, e.g. β-ecdysone. It is suggested that the hormone was rapidly converted to a metabolite which did not correspond with the apolar 3-α-dehydro-ecdysone, as was initially speculated.

Original languageEnglish (US)
Pages (from-to)433-439
Number of pages7
JournalInsect Biochemistry
Issue number4
StatePublished - 1976


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