Morphology, cytoskeletal organization, and myosin dynamics of mouse embryonic fibroblasts cultured on nanofibrillar surfaces

Ijaz Ahmed, Abdul S. Ponery, Alam Nur-E-Kamal, Jabeen Kamal, Adam S. Meshel, Michael Sheetz, Melvin Schindler, Sally Meiners

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Growth of cells in tissue culture is generally performed on two-dimensional (2D) surfaces composed of polystyrene or glass. Recent work, however, has shown that such 2D cultures are incomplete and do not adequately represent the physical characteristics of native extracellular matrix (ECM)/basement membrane (BM), namely dimensionality, compliance, fibrillarity, and porosity. In the current study, a three-dimensional (3D) nanofibrillar surface composed of electrospun polyamide nanofibers was utilized to mimic the topology and physical structure of ECM/BM. Additional chemical cues were incorporated into the nanofibrillar matrix by coating the surfaces with fibronectin, collagen I, or laminin-1. Results from the current study show an enhanced response of primary mouse embryonic fibroblasts (MEFs) to culture on nanofibrillar surfaces with more dramatic changes in cell spreading and reorganization of the cytoskeleton than previously observed for established cell lines. In addition, the cells cultured on nanofibrillar and 2D surfaces exhibited differential responses to the specific ECM/BM coatings. The localization and activity of myosin II-B for MEFs cultured on nanofibers was also compared. A dynamic redistribution of myosin II-B was observed within membrane protrusions. This was previously described for cells associated with nanofibers composed of collagen I but not for cells attached to 2D surfaces coated with monomeric collagen. These results provide further evidence that nanofibrillar surfaces offer a significantly different environment for cells than 2D substrates.

Original languageEnglish (US)
Pages (from-to)241-249
Number of pages9
JournalMolecular and Cellular Biochemistry
Volume301
Issue number1-2
DOIs
StatePublished - Jul 1 2007
Externally publishedYes

Fingerprint

Fibroblasts
Myosins
Nanofibers
Nonmuscle Myosin Type IIB
Basement Membrane
Myosin Type II
Extracellular Matrix
Collagen
Porosity
Polystyrenes
Nylons
Cells
Cytoskeleton
Fibronectins
Compliance
Coatings
Tissue culture
Glass
Cues
Cultured Cells

Keywords

  • Collagen I
  • Extra cellular matrix
  • Fibronectin
  • Laminin-1
  • Mouse embryonic fibroblasts
  • MyosinII-B
  • Nanofibers
  • Nanofibrillar surfaces

ASJC Scopus subject areas

  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology

Cite this

Morphology, cytoskeletal organization, and myosin dynamics of mouse embryonic fibroblasts cultured on nanofibrillar surfaces. / Ahmed, Ijaz; Ponery, Abdul S.; Nur-E-Kamal, Alam; Kamal, Jabeen; Meshel, Adam S.; Sheetz, Michael; Schindler, Melvin; Meiners, Sally.

In: Molecular and Cellular Biochemistry, Vol. 301, No. 1-2, 01.07.2007, p. 241-249.

Research output: Contribution to journalArticle

Ahmed, Ijaz ; Ponery, Abdul S. ; Nur-E-Kamal, Alam ; Kamal, Jabeen ; Meshel, Adam S. ; Sheetz, Michael ; Schindler, Melvin ; Meiners, Sally. / Morphology, cytoskeletal organization, and myosin dynamics of mouse embryonic fibroblasts cultured on nanofibrillar surfaces. In: Molecular and Cellular Biochemistry. 2007 ; Vol. 301, No. 1-2. pp. 241-249.
@article{fd3f03117e5644df9b816ed1166c41da,
title = "Morphology, cytoskeletal organization, and myosin dynamics of mouse embryonic fibroblasts cultured on nanofibrillar surfaces",
abstract = "Growth of cells in tissue culture is generally performed on two-dimensional (2D) surfaces composed of polystyrene or glass. Recent work, however, has shown that such 2D cultures are incomplete and do not adequately represent the physical characteristics of native extracellular matrix (ECM)/basement membrane (BM), namely dimensionality, compliance, fibrillarity, and porosity. In the current study, a three-dimensional (3D) nanofibrillar surface composed of electrospun polyamide nanofibers was utilized to mimic the topology and physical structure of ECM/BM. Additional chemical cues were incorporated into the nanofibrillar matrix by coating the surfaces with fibronectin, collagen I, or laminin-1. Results from the current study show an enhanced response of primary mouse embryonic fibroblasts (MEFs) to culture on nanofibrillar surfaces with more dramatic changes in cell spreading and reorganization of the cytoskeleton than previously observed for established cell lines. In addition, the cells cultured on nanofibrillar and 2D surfaces exhibited differential responses to the specific ECM/BM coatings. The localization and activity of myosin II-B for MEFs cultured on nanofibers was also compared. A dynamic redistribution of myosin II-B was observed within membrane protrusions. This was previously described for cells associated with nanofibers composed of collagen I but not for cells attached to 2D surfaces coated with monomeric collagen. These results provide further evidence that nanofibrillar surfaces offer a significantly different environment for cells than 2D substrates.",
keywords = "Collagen I, Extra cellular matrix, Fibronectin, Laminin-1, Mouse embryonic fibroblasts, MyosinII-B, Nanofibers, Nanofibrillar surfaces",
author = "Ijaz Ahmed and Ponery, {Abdul S.} and Alam Nur-E-Kamal and Jabeen Kamal and Meshel, {Adam S.} and Michael Sheetz and Melvin Schindler and Sally Meiners",
year = "2007",
month = "7",
day = "1",
doi = "10.1007/s11010-007-9417-6",
language = "English (US)",
volume = "301",
pages = "241--249",
journal = "Molecular and Cellular Biochemistry",
issn = "0300-8177",
publisher = "Springer Netherlands",
number = "1-2",

}

TY - JOUR

T1 - Morphology, cytoskeletal organization, and myosin dynamics of mouse embryonic fibroblasts cultured on nanofibrillar surfaces

AU - Ahmed, Ijaz

AU - Ponery, Abdul S.

AU - Nur-E-Kamal, Alam

AU - Kamal, Jabeen

AU - Meshel, Adam S.

AU - Sheetz, Michael

AU - Schindler, Melvin

AU - Meiners, Sally

PY - 2007/7/1

Y1 - 2007/7/1

N2 - Growth of cells in tissue culture is generally performed on two-dimensional (2D) surfaces composed of polystyrene or glass. Recent work, however, has shown that such 2D cultures are incomplete and do not adequately represent the physical characteristics of native extracellular matrix (ECM)/basement membrane (BM), namely dimensionality, compliance, fibrillarity, and porosity. In the current study, a three-dimensional (3D) nanofibrillar surface composed of electrospun polyamide nanofibers was utilized to mimic the topology and physical structure of ECM/BM. Additional chemical cues were incorporated into the nanofibrillar matrix by coating the surfaces with fibronectin, collagen I, or laminin-1. Results from the current study show an enhanced response of primary mouse embryonic fibroblasts (MEFs) to culture on nanofibrillar surfaces with more dramatic changes in cell spreading and reorganization of the cytoskeleton than previously observed for established cell lines. In addition, the cells cultured on nanofibrillar and 2D surfaces exhibited differential responses to the specific ECM/BM coatings. The localization and activity of myosin II-B for MEFs cultured on nanofibers was also compared. A dynamic redistribution of myosin II-B was observed within membrane protrusions. This was previously described for cells associated with nanofibers composed of collagen I but not for cells attached to 2D surfaces coated with monomeric collagen. These results provide further evidence that nanofibrillar surfaces offer a significantly different environment for cells than 2D substrates.

AB - Growth of cells in tissue culture is generally performed on two-dimensional (2D) surfaces composed of polystyrene or glass. Recent work, however, has shown that such 2D cultures are incomplete and do not adequately represent the physical characteristics of native extracellular matrix (ECM)/basement membrane (BM), namely dimensionality, compliance, fibrillarity, and porosity. In the current study, a three-dimensional (3D) nanofibrillar surface composed of electrospun polyamide nanofibers was utilized to mimic the topology and physical structure of ECM/BM. Additional chemical cues were incorporated into the nanofibrillar matrix by coating the surfaces with fibronectin, collagen I, or laminin-1. Results from the current study show an enhanced response of primary mouse embryonic fibroblasts (MEFs) to culture on nanofibrillar surfaces with more dramatic changes in cell spreading and reorganization of the cytoskeleton than previously observed for established cell lines. In addition, the cells cultured on nanofibrillar and 2D surfaces exhibited differential responses to the specific ECM/BM coatings. The localization and activity of myosin II-B for MEFs cultured on nanofibers was also compared. A dynamic redistribution of myosin II-B was observed within membrane protrusions. This was previously described for cells associated with nanofibers composed of collagen I but not for cells attached to 2D surfaces coated with monomeric collagen. These results provide further evidence that nanofibrillar surfaces offer a significantly different environment for cells than 2D substrates.

KW - Collagen I

KW - Extra cellular matrix

KW - Fibronectin

KW - Laminin-1

KW - Mouse embryonic fibroblasts

KW - MyosinII-B

KW - Nanofibers

KW - Nanofibrillar surfaces

UR - http://www.scopus.com/inward/record.url?scp=34250189537&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34250189537&partnerID=8YFLogxK

U2 - 10.1007/s11010-007-9417-6

DO - 10.1007/s11010-007-9417-6

M3 - Article

VL - 301

SP - 241

EP - 249

JO - Molecular and Cellular Biochemistry

JF - Molecular and Cellular Biochemistry

SN - 0300-8177

IS - 1-2

ER -