TY - JOUR
T1 - Mosquito cells persistently infected with dengue virus produce viral particles with host-dependent replication
AU - Reyes-Ruiz, José Manuel
AU - Osuna-Ramos, Juan Fidel
AU - Bautista-Carbajal, Patricia
AU - Jaworski, Elizabeth
AU - Soto-Acosta, Rubén
AU - Cervantes-Salazar, Margot
AU - Angel-Ambrocio, Antonio H.
AU - Castillo-Munguía, Juan Pablo
AU - Chávez-Munguía, Bibiana
AU - De Nova-Ocampo, Mónica
AU - Routh, Andrew
AU - del Ángel, Rosa María
AU - Salas-Benito, Juan Santiago
N1 - Funding Information:
We thank Mariana Salas-Benito for the maintenance of cell lines and mice, and Anel E. Lagunes-Guillén, Matilde García-Espitia, Fernando Medina, and Jaime Zarco for their technical assistance. We would like to thank Dr. Juan E. Ludert from CINVESTAV (Mexico) for their contributions to manuscript reviewing. This project was supported by Secretaría de Investigación y Posgrado ( SIP 20151372 ) of Instituto Politécnico Nacional, Consejo Nacional de Ciencia y Tecnología ( CONACYT , Mexico) Grant 220824 , and Fundación Miguel Alemán . Drs. Salas-Benito and De Nova-Ocampo have fellowships from Estímulo al Desempeño de los Investigadores ( EDI ) and Comisión de Operación y Fomento a las Actividades Académicas ( COFAA ) from Instituto Politécnico Nacional. José Manuel Reyes-Ruiz, Juan Fidel Osuna-Ramos, and Juan Pablo Castillo-Munguía received scholarships from CONACYT.
Funding Information:
We thank Mariana Salas-Benito for the maintenance of cell lines and mice, and Anel E. Lagunes-Guillén, Matilde García-Espitia, Fernando Medina, and Jaime Zarco for their technical assistance. We would like to thank Dr. Juan E. Ludert from CINVESTAV (Mexico) for their contributions to manuscript reviewing. This project was supported by Secretaría de Investigación y Posgrado (SIP 20151372) of Instituto Politécnico Nacional, Consejo Nacional de Ciencia y Tecnología (CONACYT, Mexico) Grant 220824, and Fundación Miguel Alemán. Drs. Salas-Benito and De Nova-Ocampo have fellowships from Estímulo al Desempeño de los Investigadores (EDI) and Comisión de Operación y Fomento a las Actividades Académicas (COFAA) from Instituto Politécnico Nacional. José Manuel Reyes-Ruiz, Juan Fidel Osuna-Ramos, and Juan Pablo Castillo-Munguía received scholarships from CONACYT.
PY - 2019/5
Y1 - 2019/5
N2 - Dengue viruses (DENV) are important arboviruses that can establish a persistent infection in its mosquito vector Aedes. Mosquitoes have a short lifetime in nature which makes trying to study the processes that take place during persistent viral infections in vivo. Therefore, C6/36 cells have been used to study this type of infection. C6/36 cells persistently infected with DENV 2 produce virions that cannot infect BHK -21 cells. We hypothesized that the following passages in mosquito cells have a deleterious impact on DENV fitness in vertebrate cells. Here, we demonstrated that the viral particles released from persistently infected cells were infectious to mosquito but not to vertebrate cells. This host restriction occurs at the replication level and is associated with several mutations in the DENV genome. In summary, our findings provide new information about viral replication fitness in a host-dependent manner.
AB - Dengue viruses (DENV) are important arboviruses that can establish a persistent infection in its mosquito vector Aedes. Mosquitoes have a short lifetime in nature which makes trying to study the processes that take place during persistent viral infections in vivo. Therefore, C6/36 cells have been used to study this type of infection. C6/36 cells persistently infected with DENV 2 produce virions that cannot infect BHK -21 cells. We hypothesized that the following passages in mosquito cells have a deleterious impact on DENV fitness in vertebrate cells. Here, we demonstrated that the viral particles released from persistently infected cells were infectious to mosquito but not to vertebrate cells. This host restriction occurs at the replication level and is associated with several mutations in the DENV genome. In summary, our findings provide new information about viral replication fitness in a host-dependent manner.
KW - Dengue viruses
KW - Host adaptation
KW - Mosquito cells
KW - Persistent viral infection
KW - Viral fitness
UR - http://www.scopus.com/inward/record.url?scp=85062725676&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85062725676&partnerID=8YFLogxK
U2 - 10.1016/j.virol.2019.02.018
DO - 10.1016/j.virol.2019.02.018
M3 - Article
C2 - 30844508
AN - SCOPUS:85062725676
SN - 0042-6822
VL - 531
SP - 1
EP - 18
JO - Virology
JF - Virology
ER -