@article{129afc0b90774484a5434c6cb8f1302a,
title = "Mouse-adapted SARS-CoV-2 protects animals from lethal SARS-CoV challenge",
abstract = "The emergence of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has resulted in a pandemic causing significant damage to public health and the economy. Efforts to understand the mechanisms of Coronavirus Disease 2019 (COVID-19): have been hamered by the lack of robust mouse models. To overcome this barrier, we used a reverse genetic system to generate a mouse-adapted strain of SARS-CoV-2. Incorporating key mutations found in SARS-CoV-2 variants, this model recapitulates critical elements of human infection including viral replication in the lung, immune cell infiltration, and significant in vivo disease. Importantly, mouse adaptation of SARS-CoV-2 does not impair replication in human airway cells and maintains antigenicity similar to human SARS-CoV-2 strains. Coupled with the incorporation of mutations found in variants of concern, CMA3p20 offers several advantages over other mouse-adapted SARS-CoV-2 strains. Using this model, we demonstrate that SARS-CoV-2–infected mice are protected from lethal challenge with the original Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV), suggesting immunity from heterologous Coronavirus (CoV) strains. Together, the results highlight the use of this mouse model for further study of SARS-CoV-2 infection and disease.",
author = "Antonio Muruato and Vu, {Michelle N.} and Johnson, {Bryan A.} and Davis-Gardner, {Meredith E.} and Abigail Vanderheiden and Kumari Lokugamage and Craig Schindewolf and Crocquet-Valdes, {Patricia A.} and Langsjoen, {Rose M.} and Plante, {Jessica A.} and Plante, {Kenneth S.} and Weaver, {Scott C.} and Kari Debbink and Routh, {Andrew L.} and David Walker and Suthar, {Mehul S.} and Shi, {Pei Yong} and Xuping Xie and Menachery, {Vineet D.}",
note = "Funding Information: Research was supported by grants from NIAID of the NIH to (AI153602 and 1R21AI145400 to VDM to P-YS; R24AI120942 (WRCEVA) to SCW, P51OD011132, R56 AI147623 and U19AI090023 to MSS. AEM is supported by a Clinical and Translational Science Award NRSA (TL1) Training Core (TL1TR001440) from NIH. ALR was supported by an Institute of Human Infection and Immunity at UTMB COVID-19 Research Fund. Research was also supported by STARs Award provided by the University of Texas System to VDM, and trainee funding provided by the McLaughlin Fellowship Fund at UTMB. P-YS was also supported by CDC grant for the Western Gulf Center of Excellence for Vector-Borne Diseases, and awards from the Sealy & Smith Foundation, Kleberg Foundation, John S. Dunn Foundation, Amon G. Carter Foundation, Gilson Longenbaugh Foundation, and Summerfield Robert Foundation. MSS was also supported by the Emory Executive Vice President for Health Affairs Synergy Fund award, the Pediatric Research Alliance Center for Childhood Infections and Vaccines and Children{\textquoteright}s Healthcare of Atlanta, COVID-Catalyst-I3 Funds from the Woodruff Health Sciences Center and Emory School of Medicine, Woodruff Health Sciences Center 2020 COVID-19 CURE Award. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.” Publisher Copyright: {\textcopyright} 2021 Muruato et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.",
year = "2021",
month = nov,
doi = "10.1371/journal.pbio.3001284",
language = "English (US)",
volume = "19",
journal = "PLoS Biology",
issn = "1544-9173",
publisher = "Public Library of Science",
number = "11",
}