Mouse Fem1b interacts with and induces ubiquitin-mediated degradation of Ankrd37

Yu Qiang Shi, Shang Ying Liao, Xin Jie Zhuang, Chun Sheng Han

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

Ankyrin repeat domain 37 (Ankrd37), a protein containing ankyrin repeats (ARs) and a putative nuclear localization signal (NLS), is highly conserved from zebrafish to humans. In mouse testes, Ankrd37 protein was initially present in the cytoplasm of elongating spermatids, and finally restricted to the nuclei of spermatozoa during spermatogenesis. Ankrd37 bound to feminization 1 homolog b (Fem1b) as indicated by yeast two-hybrid screening and co-immunoprecipitation assays. Ankrd37 facilitated the transport of Fem1b protein from cytoplasm to nuclei in co-transfected CHO cells. In addition, the protein level of Ankrd37 was decreased in a Fem1b dose-dependent manner as shown by the transfection experiments, and Ankrd37 was ubiquitinated in the presence of Fem1b. As the nematode Fem-1 has been shown to target its downstream effector TRA-1 for ubiquitin-mediated degradation, we report in the present study that mouse Fem1b targets Ankrd37 for degradation in the same manner.

Original languageEnglish (US)
Pages (from-to)153-159
Number of pages7
JournalGene
Volume485
Issue number2
DOIs
StatePublished - Oct 10 2011

Keywords

  • Ankrd37
  • Ankyrin repeat motif
  • Spermatogenesis
  • Testis
  • Ubiquitination

ASJC Scopus subject areas

  • Genetics

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