Mouse neural progenitor cells differentiate into oligodendrocytes in the brain of a knockout mouse model of Canavan disease

Sankar Surendran, Lamya S. Shihabuddin, Jennifer Clarke, Tatyana V. Taksir, Gregory R. Stewart, Geoffrey Parsons, Wendy Yang, Stephen K. Tyring, Kimberlee Michals-Matalon, Reuben Matalon

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Canavan disease (CD) is an autosomal recessive disorder that leads to spongy degeneration in the white matter of the brain. Aspartoacylase (ASPA) synthesizing cells, oligodendrocytes, are lost in CD. Transplantation of neural progenitor cells (NPCs) offers an interesting therapeutic approach for treating neurodegenerative diseases by replacing the lost cells. Therefore, the NPCs transplantation to the brain of the CD mouse was studied. Injection of mouse NPCs to the striatum and cerebellum of juvenile CD mouse showed numerous BrdU positive cells at 1 month after injection. The same result was also observed in the adult CD mouse brain after 5 weeks of post-transplantation period. The implanted cells differentiated into oligodendrocytes and fibrous astrocytes, as observed using glial cell marker. This is the first report to describe the survival, distribution and differentiation of NPCs within the brain of CD mouse and a first step toward the potential clinical use of cell therapy to treat CD.

Original languageEnglish (US)
Pages (from-to)19-27
Number of pages9
JournalDevelopmental Brain Research
Volume153
Issue number1
DOIs
StatePublished - Oct 15 2004
Externally publishedYes

Fingerprint

Canavan Disease
Oligodendroglia
Knockout Mice
Stem Cells
Brain
Brain Diseases
Transplantation
Injections
Cell Transplantation
Bromodeoxyuridine
Cell- and Tissue-Based Therapy
Neuroglia
Astrocytes
Neurodegenerative Diseases
Cerebellum

Keywords

  • Astrocyte
  • Canavan disease
  • Cell therapy
  • Knockout mouse
  • Neural progenitor cell
  • Oligodendrocyte
  • Retrovirus

ASJC Scopus subject areas

  • Developmental Biology
  • Developmental Neuroscience

Cite this

Mouse neural progenitor cells differentiate into oligodendrocytes in the brain of a knockout mouse model of Canavan disease. / Surendran, Sankar; Shihabuddin, Lamya S.; Clarke, Jennifer; Taksir, Tatyana V.; Stewart, Gregory R.; Parsons, Geoffrey; Yang, Wendy; Tyring, Stephen K.; Michals-Matalon, Kimberlee; Matalon, Reuben.

In: Developmental Brain Research, Vol. 153, No. 1, 15.10.2004, p. 19-27.

Research output: Contribution to journalArticle

Surendran, S, Shihabuddin, LS, Clarke, J, Taksir, TV, Stewart, GR, Parsons, G, Yang, W, Tyring, SK, Michals-Matalon, K & Matalon, R 2004, 'Mouse neural progenitor cells differentiate into oligodendrocytes in the brain of a knockout mouse model of Canavan disease', Developmental Brain Research, vol. 153, no. 1, pp. 19-27. https://doi.org/10.1016/j.devbrainres.2004.07.003
Surendran, Sankar ; Shihabuddin, Lamya S. ; Clarke, Jennifer ; Taksir, Tatyana V. ; Stewart, Gregory R. ; Parsons, Geoffrey ; Yang, Wendy ; Tyring, Stephen K. ; Michals-Matalon, Kimberlee ; Matalon, Reuben. / Mouse neural progenitor cells differentiate into oligodendrocytes in the brain of a knockout mouse model of Canavan disease. In: Developmental Brain Research. 2004 ; Vol. 153, No. 1. pp. 19-27.
@article{eadc969d96fa4e7b8f59afbdde118132,
title = "Mouse neural progenitor cells differentiate into oligodendrocytes in the brain of a knockout mouse model of Canavan disease",
abstract = "Canavan disease (CD) is an autosomal recessive disorder that leads to spongy degeneration in the white matter of the brain. Aspartoacylase (ASPA) synthesizing cells, oligodendrocytes, are lost in CD. Transplantation of neural progenitor cells (NPCs) offers an interesting therapeutic approach for treating neurodegenerative diseases by replacing the lost cells. Therefore, the NPCs transplantation to the brain of the CD mouse was studied. Injection of mouse NPCs to the striatum and cerebellum of juvenile CD mouse showed numerous BrdU positive cells at 1 month after injection. The same result was also observed in the adult CD mouse brain after 5 weeks of post-transplantation period. The implanted cells differentiated into oligodendrocytes and fibrous astrocytes, as observed using glial cell marker. This is the first report to describe the survival, distribution and differentiation of NPCs within the brain of CD mouse and a first step toward the potential clinical use of cell therapy to treat CD.",
keywords = "Astrocyte, Canavan disease, Cell therapy, Knockout mouse, Neural progenitor cell, Oligodendrocyte, Retrovirus",
author = "Sankar Surendran and Shihabuddin, {Lamya S.} and Jennifer Clarke and Taksir, {Tatyana V.} and Stewart, {Gregory R.} and Geoffrey Parsons and Wendy Yang and Tyring, {Stephen K.} and Kimberlee Michals-Matalon and Reuben Matalon",
year = "2004",
month = "10",
day = "15",
doi = "10.1016/j.devbrainres.2004.07.003",
language = "English (US)",
volume = "153",
pages = "19--27",
journal = "Developmental Brain Research",
issn = "0165-3806",
publisher = "Elsevier BV",
number = "1",

}

TY - JOUR

T1 - Mouse neural progenitor cells differentiate into oligodendrocytes in the brain of a knockout mouse model of Canavan disease

AU - Surendran, Sankar

AU - Shihabuddin, Lamya S.

AU - Clarke, Jennifer

AU - Taksir, Tatyana V.

AU - Stewart, Gregory R.

AU - Parsons, Geoffrey

AU - Yang, Wendy

AU - Tyring, Stephen K.

AU - Michals-Matalon, Kimberlee

AU - Matalon, Reuben

PY - 2004/10/15

Y1 - 2004/10/15

N2 - Canavan disease (CD) is an autosomal recessive disorder that leads to spongy degeneration in the white matter of the brain. Aspartoacylase (ASPA) synthesizing cells, oligodendrocytes, are lost in CD. Transplantation of neural progenitor cells (NPCs) offers an interesting therapeutic approach for treating neurodegenerative diseases by replacing the lost cells. Therefore, the NPCs transplantation to the brain of the CD mouse was studied. Injection of mouse NPCs to the striatum and cerebellum of juvenile CD mouse showed numerous BrdU positive cells at 1 month after injection. The same result was also observed in the adult CD mouse brain after 5 weeks of post-transplantation period. The implanted cells differentiated into oligodendrocytes and fibrous astrocytes, as observed using glial cell marker. This is the first report to describe the survival, distribution and differentiation of NPCs within the brain of CD mouse and a first step toward the potential clinical use of cell therapy to treat CD.

AB - Canavan disease (CD) is an autosomal recessive disorder that leads to spongy degeneration in the white matter of the brain. Aspartoacylase (ASPA) synthesizing cells, oligodendrocytes, are lost in CD. Transplantation of neural progenitor cells (NPCs) offers an interesting therapeutic approach for treating neurodegenerative diseases by replacing the lost cells. Therefore, the NPCs transplantation to the brain of the CD mouse was studied. Injection of mouse NPCs to the striatum and cerebellum of juvenile CD mouse showed numerous BrdU positive cells at 1 month after injection. The same result was also observed in the adult CD mouse brain after 5 weeks of post-transplantation period. The implanted cells differentiated into oligodendrocytes and fibrous astrocytes, as observed using glial cell marker. This is the first report to describe the survival, distribution and differentiation of NPCs within the brain of CD mouse and a first step toward the potential clinical use of cell therapy to treat CD.

KW - Astrocyte

KW - Canavan disease

KW - Cell therapy

KW - Knockout mouse

KW - Neural progenitor cell

KW - Oligodendrocyte

KW - Retrovirus

UR - http://www.scopus.com/inward/record.url?scp=4644292063&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=4644292063&partnerID=8YFLogxK

U2 - 10.1016/j.devbrainres.2004.07.003

DO - 10.1016/j.devbrainres.2004.07.003

M3 - Article

C2 - 15464214

AN - SCOPUS:4644292063

VL - 153

SP - 19

EP - 27

JO - Developmental Brain Research

JF - Developmental Brain Research

SN - 0165-3806

IS - 1

ER -