Movement of myosin-coated fluorescent beads on actin cables in vitro

Michael Sheetz, James A. Spudich

Research output: Contribution to journalReview article

300 Citations (Scopus)

Abstract

Myosin-coated fluorescent beads are observed to move unidirectionally along organized actin filament arrays in the alga, Nitella, with an average velocity similar to in vivo rates of movement in muscle and other cells. The myosin produces the motive force, as the movement is ATP dependent and is blocked by inactivation of the myosin heads. The movement, which occurs in the absence of bipolar thick filaments, provides a quantitative assay for myosin motility.

Original languageEnglish (US)
Pages (from-to)31-35
Number of pages5
JournalNature
Volume303
Issue number5912
DOIs
StatePublished - Dec 1 1983
Externally publishedYes

Fingerprint

Myosins
Actins
Nitella
Actin Cytoskeleton
Muscle Cells
Adenosine Triphosphate
In Vitro Techniques

ASJC Scopus subject areas

  • General

Cite this

Movement of myosin-coated fluorescent beads on actin cables in vitro. / Sheetz, Michael; Spudich, James A.

In: Nature, Vol. 303, No. 5912, 01.12.1983, p. 31-35.

Research output: Contribution to journalReview article

Sheetz, Michael ; Spudich, James A. / Movement of myosin-coated fluorescent beads on actin cables in vitro. In: Nature. 1983 ; Vol. 303, No. 5912. pp. 31-35.
@article{ffb6b1ffb3f14353922c57f9df4cb03e,
title = "Movement of myosin-coated fluorescent beads on actin cables in vitro",
abstract = "Myosin-coated fluorescent beads are observed to move unidirectionally along organized actin filament arrays in the alga, Nitella, with an average velocity similar to in vivo rates of movement in muscle and other cells. The myosin produces the motive force, as the movement is ATP dependent and is blocked by inactivation of the myosin heads. The movement, which occurs in the absence of bipolar thick filaments, provides a quantitative assay for myosin motility.",
author = "Michael Sheetz and Spudich, {James A.}",
year = "1983",
month = "12",
day = "1",
doi = "10.1038/303031a0",
language = "English (US)",
volume = "303",
pages = "31--35",
journal = "Nature",
issn = "0028-0836",
publisher = "Nature Publishing Group",
number = "5912",

}

TY - JOUR

T1 - Movement of myosin-coated fluorescent beads on actin cables in vitro

AU - Sheetz, Michael

AU - Spudich, James A.

PY - 1983/12/1

Y1 - 1983/12/1

N2 - Myosin-coated fluorescent beads are observed to move unidirectionally along organized actin filament arrays in the alga, Nitella, with an average velocity similar to in vivo rates of movement in muscle and other cells. The myosin produces the motive force, as the movement is ATP dependent and is blocked by inactivation of the myosin heads. The movement, which occurs in the absence of bipolar thick filaments, provides a quantitative assay for myosin motility.

AB - Myosin-coated fluorescent beads are observed to move unidirectionally along organized actin filament arrays in the alga, Nitella, with an average velocity similar to in vivo rates of movement in muscle and other cells. The myosin produces the motive force, as the movement is ATP dependent and is blocked by inactivation of the myosin heads. The movement, which occurs in the absence of bipolar thick filaments, provides a quantitative assay for myosin motility.

UR - http://www.scopus.com/inward/record.url?scp=0020586332&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020586332&partnerID=8YFLogxK

U2 - 10.1038/303031a0

DO - 10.1038/303031a0

M3 - Review article

C2 - 6682486

AN - SCOPUS:0020586332

VL - 303

SP - 31

EP - 35

JO - Nature

JF - Nature

SN - 0028-0836

IS - 5912

ER -