Movement of myosin‐coated structures on actin cables

Michael Sheetz, James A. Spudich

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Myosin‐coated spheres from 0.6 to 120 μm in diameter move in vitro on a substratum of polar arrays of actin cables derived from the alga Nitella. The force for this movement is provided by skeletal muscle myosin since it is ATP‐dependent, and N‐ethylmaleimide (NEM) inactivation of the myosin blocks movement. These observations demonstrate that attachment of myosin in a random orientation to structures will enable those structures to move along polar arrays of actin filaments.

Original languageEnglish (US)
Pages (from-to)485-489
Number of pages5
JournalCell Motility
Volume3
Issue number5
DOIs
StatePublished - Jan 1 1983
Externally publishedYes

Fingerprint

Myosins
Actins
Nitella
Skeletal Muscle Myosins
Actin Cytoskeleton
In Vitro Techniques

Keywords

  • actin
  • cell motility
  • myosin
  • vesicle transport

ASJC Scopus subject areas

  • Cell Biology

Cite this

Movement of myosin‐coated structures on actin cables. / Sheetz, Michael; Spudich, James A.

In: Cell Motility, Vol. 3, No. 5, 01.01.1983, p. 485-489.

Research output: Contribution to journalArticle

Sheetz, Michael ; Spudich, James A. / Movement of myosin‐coated structures on actin cables. In: Cell Motility. 1983 ; Vol. 3, No. 5. pp. 485-489.
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