Movement of organelles along filaments dissociated from the axoplasm of the squid giant axon

Ronald D. Vale, Bruce J. Schnapp, Thomas S. Reese, Michael Sheetz

Research output: Contribution to journalArticle

188 Citations (Scopus)

Abstract

Cytoplasmic filaments, separated from the axoplasm of the squid giant axon and visualized by video-enhanced differential interference contrast microscopy, support the directed movement of organelles in the presence of ATP All organelles, regardless of size, move continuously along isolated transport filaments at 2.2 ± 0.2 μm/sec. In the intact axoplasm, however, movements of the larger organelles are slow and saltatory. These movements may reflect a resistance to movement imposed by the intact axoplasm. The uniform rate of all organelles along isolated transport filaments suggests that a single type of molecular motor powers fast axonal transport. Organelles can attach to and move along more than one filament at a time, suggesting that organelles have multiple binding sites for this motor.

Original languageEnglish (US)
Pages (from-to)449-454
Number of pages6
JournalCell
Volume40
Issue number2
DOIs
StatePublished - Jan 1 1985
Externally publishedYes

Fingerprint

Decapodiformes
Organelles
Axons
Microscopic examination
Adenosine Triphosphate
Binding Sites
Organelle Size
Interference Microscopy
Axonal Transport
Cytoskeleton

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Medicine(all)

Cite this

Movement of organelles along filaments dissociated from the axoplasm of the squid giant axon. / Vale, Ronald D.; Schnapp, Bruce J.; Reese, Thomas S.; Sheetz, Michael.

In: Cell, Vol. 40, No. 2, 01.01.1985, p. 449-454.

Research output: Contribution to journalArticle

Vale, Ronald D. ; Schnapp, Bruce J. ; Reese, Thomas S. ; Sheetz, Michael. / Movement of organelles along filaments dissociated from the axoplasm of the squid giant axon. In: Cell. 1985 ; Vol. 40, No. 2. pp. 449-454.
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