MRI assessment of ischemic liver after intraportal islet transplantation

Naoaki Sakata, Pete Hayes, Annie Tan, Nathaniel K. Chan, John MacE, Ricardo Peverini, Lawrence Sowers, William J. Pearce, Richard Chinnock, Andre Obenaus, Eba Hathout

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

BACKGROUND.: There is a recent focus on embolization of the portal vein by transplanted islets as a major cause of early graft loss. The resultant ischemia causes necrosis or apoptosis of cells within the liver. Thus, noninvasive assessment of the liver receiving the islet transplant is important to evaluate the status islet grafts. METHODS.: This study used noninvasive magnetic resonance imaging (MRI) for assessment of the posttransplant ischemic liver. Syngeneic islets in streprozotocin-induced diabetic mice were used. MRI and morphological liver assessments were performed at 0, 2, and 28 days after transplantation. Histologic assessment of insulin, hypoxia induced factor 1-α, and apoptosis were undertaken at similar time points. RESULTS.: Ischemic/necrotic regions in the liver were detected by MRI at 2 days but not at 28 days after transplantation and were confirmed histologically. Liver injury was quantified from high intensity areas on T2-weighted images. Insulin release peaked 2 days after transplantation. CONCLUSION.: Onset and reversal of liver ischemia due to intraportal islet transplantation are detectable using T2-weighted MRI. These changes coincide with periods of maximum insulin release likely due to partial islet destruction. We propose that MRI, as a noninvasive monitor of graft-related ischemia, may be useful in assessment of liver and islet engraftment after intraportal islet transplantation in a clinical setting.

Original languageEnglish (US)
Pages (from-to)825-830
Number of pages6
JournalTransplantation
Volume87
Issue number6
DOIs
StatePublished - Mar 27 2009
Externally publishedYes

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Islets of Langerhans Transplantation
Magnetic Resonance Imaging
Liver
Transplants
Ischemia
Transplantation
Insulin
Apoptosis
Portal Vein
Necrosis
Wounds and Injuries

Keywords

  • Imaging
  • Intraportal transplantation
  • Islet transplantation
  • Liver ischemia

ASJC Scopus subject areas

  • Transplantation

Cite this

Sakata, N., Hayes, P., Tan, A., Chan, N. K., MacE, J., Peverini, R., ... Hathout, E. (2009). MRI assessment of ischemic liver after intraportal islet transplantation. Transplantation, 87(6), 825-830. https://doi.org/10.1097/TP.0b013e318199c7d2

MRI assessment of ischemic liver after intraportal islet transplantation. / Sakata, Naoaki; Hayes, Pete; Tan, Annie; Chan, Nathaniel K.; MacE, John; Peverini, Ricardo; Sowers, Lawrence; Pearce, William J.; Chinnock, Richard; Obenaus, Andre; Hathout, Eba.

In: Transplantation, Vol. 87, No. 6, 27.03.2009, p. 825-830.

Research output: Contribution to journalArticle

Sakata, N, Hayes, P, Tan, A, Chan, NK, MacE, J, Peverini, R, Sowers, L, Pearce, WJ, Chinnock, R, Obenaus, A & Hathout, E 2009, 'MRI assessment of ischemic liver after intraportal islet transplantation', Transplantation, vol. 87, no. 6, pp. 825-830. https://doi.org/10.1097/TP.0b013e318199c7d2
Sakata N, Hayes P, Tan A, Chan NK, MacE J, Peverini R et al. MRI assessment of ischemic liver after intraportal islet transplantation. Transplantation. 2009 Mar 27;87(6):825-830. https://doi.org/10.1097/TP.0b013e318199c7d2
Sakata, Naoaki ; Hayes, Pete ; Tan, Annie ; Chan, Nathaniel K. ; MacE, John ; Peverini, Ricardo ; Sowers, Lawrence ; Pearce, William J. ; Chinnock, Richard ; Obenaus, Andre ; Hathout, Eba. / MRI assessment of ischemic liver after intraportal islet transplantation. In: Transplantation. 2009 ; Vol. 87, No. 6. pp. 825-830.
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