Multi-well plate immunoassays for measuring signaling protein activations/deactivations and membrane vs. intracellular receptor levels

Cheryl S. Watson, Yow Jiun Jeng, Nataliya N. Bulayeva, Celeste C. Finnerty, Luke Y. Koong, Dragoslaca Zivadinovic, Rebecca A. Alyea, Terumi Midoro-Horiuti, Randall M. Goldblum, Noelle C. Anastasio, Kathryn A. Cunningham, Patricia K. Seitz, Thressa D. Smith

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

We developed fixed-cell multi-well plate immunoassays that increase the throughput and ease of quantification for questions formerly assessed by immunoblot scanning. The assays make use of the now abundant antibodies designed to recognize receptor subtypes and posttranslationally modified signaling proteins. By optimizing permeabilization and fixation conditions, mainly based on specific cell types, the assay can be adapted to the study of many different antigens of importance to hormonal and neurotransmitter s ignaling scenarios.

Original languageEnglish (US)
Pages (from-to)123-133
Number of pages11
JournalMethods in Molecular Biology
Volume1204
DOIs
StatePublished - Jan 1 2014

Keywords

  • Cells
  • Immunoblots
  • Posttranslational modifications
  • Quantification
  • Throughput
  • Tissues

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Fingerprint Dive into the research topics of 'Multi-well plate immunoassays for measuring signaling protein activations/deactivations and membrane vs. intracellular receptor levels'. Together they form a unique fingerprint.

  • Cite this