Multi-well plate immunoassays for measuring signaling protein activations/deactivations and membrane vs. intracellular receptor levels

Cheryl S. Watson, Yow Jiun Jeng, Nataliya N. Bulayeva, Celeste Finnerty, Luke Y. Koong, Dragoslaca Zivadinovic, Rebecca A. Alyea, Terumi Midoro-Horiuti, Randall M. Goldblum, Noelle Anastasio, Kathryn Cunningham, Patricia K. Seitz, Thressa D. Smith

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We developed fixed-cell multi-well plate immunoassays that increase the throughput and ease of quantification for questions formerly assessed by immunoblot scanning. The assays make use of the now abundant antibodies designed to recognize receptor subtypes and posttranslationally modified signaling proteins. By optimizing permeabilization and fixation conditions, mainly based on specific cell types, the assay can be adapted to the study of many different antigens of importance to hormonal and neurotransmitter s ignaling scenarios.

Original languageEnglish
Pages (from-to)123-133
Number of pages11
JournalMethods in molecular biology (Clifton, N.J.)
Volume1204
DOIs
StatePublished - 2014
Externally publishedYes

Fingerprint

Immunoassay
Membranes
Neurotransmitter Agents
Proteins
Antigens
Antibodies

Keywords

  • Cells
  • Immunoblots
  • Posttranslational modifications
  • Quantification
  • Throughput
  • Tissues

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Multi-well plate immunoassays for measuring signaling protein activations/deactivations and membrane vs. intracellular receptor levels. / Watson, Cheryl S.; Jeng, Yow Jiun; Bulayeva, Nataliya N.; Finnerty, Celeste; Koong, Luke Y.; Zivadinovic, Dragoslaca; Alyea, Rebecca A.; Midoro-Horiuti, Terumi; Goldblum, Randall M.; Anastasio, Noelle; Cunningham, Kathryn; Seitz, Patricia K.; Smith, Thressa D.

In: Methods in molecular biology (Clifton, N.J.), Vol. 1204, 2014, p. 123-133.

Research output: Contribution to journalArticle

Watson, Cheryl S. ; Jeng, Yow Jiun ; Bulayeva, Nataliya N. ; Finnerty, Celeste ; Koong, Luke Y. ; Zivadinovic, Dragoslaca ; Alyea, Rebecca A. ; Midoro-Horiuti, Terumi ; Goldblum, Randall M. ; Anastasio, Noelle ; Cunningham, Kathryn ; Seitz, Patricia K. ; Smith, Thressa D. / Multi-well plate immunoassays for measuring signaling protein activations/deactivations and membrane vs. intracellular receptor levels. In: Methods in molecular biology (Clifton, N.J.). 2014 ; Vol. 1204. pp. 123-133.
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AU - Finnerty, Celeste

AU - Koong, Luke Y.

AU - Zivadinovic, Dragoslaca

AU - Alyea, Rebecca A.

AU - Midoro-Horiuti, Terumi

AU - Goldblum, Randall M.

AU - Anastasio, Noelle

AU - Cunningham, Kathryn

AU - Seitz, Patricia K.

AU - Smith, Thressa D.

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