Multidrug antimicrobial resistance and molecular detection of MCR-1 gene in salmonella species isolated from chicken

Md Bashir Uddin, SM Bayejed Hossain, Mahmudul Hasan, Mohammad Nurul Alam, Mita Debnath, Ruhena Begum, Sawrab Roy, Ahmed Harun-Al-rashid, Md Shahidur Rahman Chowdhury, Md Mahfujur Rahman, Md Mukter Hossain, Fazle Elahi, Mohammed Yousuf Elahi Chowdhury, Josef D. Järhult, Mohamed E. El Zowalaty, Syed Sayeem Uddin Ahmed

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

Colistin (polymyxin E) is widely used in animal and human medicine and is increasingly used as one of the last-resort antibiotics against Gram-negative bacilli. Due to the increased use of colistin in treating infections caused by multidrug-resistant Gram-negative bacteria, resistance to this antibiotic ought to be monitored. The study was undertaken to elucidate the molecular mechanisms, genetic relationships and phenotype correlations of colistin-resistant isolates. Here, we report the detection of the mcr-1 gene in chicken-associated Salmonella isolates in Bangladesh and its in-silico functional analysis. Out of 100 samples, 82 Salmonella spp. were isolated from chicken specimens (liver, intestine). Phenotypic disc diffusion and minimum inhibitory concentration (MIC) assay using different antimicrobial agents were performed. Salmonella isolates were characterized using PCR methods targeting genus-specific invA and mcr-1 genes with validation for the functional analysis. The majority of the tested Salmonella isolates were found resistant to colistin (92.68%), ciprofloxacin (73.17%), tigecycline (62.20%) and trimethoprim/sulfamethoxazole (60.98%). When screened using PCR, five out of ten Salmonella isolates were found to carry the mcr-1 gene. One isolate was confirmed for Salmonella enterica subsp. enterica serovar Enteritidis, and other four isolates were confirmed for Salmonella enterica subsp. enterica serovar Typhimurium. Sequencing and phylogenetic analysis revealed a divergent evolutionary relationship between the catalytic domain of Neisseria meningitidis lipooligosaccharide phosphoethanolamine transferase A (LptA) and MCR proteins, rendering them resistant to colistin. Three-dimensional homology structural analysis of MCR-1 proteins and molecular docking interactions suggested that MCR-1 and LptA share a similar substrate binding cavity, which could be validated for the functional analysis. The comprehensive molecular and in-silico analyses of the colistin resistance mcr-1 gene of Salmonella spp. of chicken origin in the present study highlight the importance of continued monitoring and surveillance for antimicrobial resistance among pathogens in food chain animals.

Original languageEnglish (US)
Article number206
Pages (from-to)1-19
Number of pages19
JournalAnimals
Volume11
Issue number1
DOIs
StatePublished - Jan 2021
Externally publishedYes

Keywords

  • Antimicrobial resistance
  • Bangladesh
  • Chicken
  • Colistin
  • Enterobacteriaceae
  • Foodborne, poultry
  • Mcr-1 gene
  • Phosphoethanolamine, LptA
  • Salmonella enterica

ASJC Scopus subject areas

  • Animal Science and Zoology
  • General Veterinary

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