Multilaboratory testing of antifungal drug combinations against Candida species and Aspergillus fumigatus: Utility of 100 percent inhibition as the endpoint

Ping Ren; Ming Luo; Shao Lin; Mahmoud A. Ghannoum; Nancy Isham; Dan J. Diekema; Michael A. Pfaller; Shawn Messer; Shawn R. Lockhart; Naureen Iqbal; Vishnu Chaturvedi

doi:10.1128/AAC.04545-14

Multilaboratory testing of antifungal drug combinations against Candida species and Aspergillus fumigatus: Utility of 100 percent inhibition as the endpoint

Ping Ren, Ming Luo, Shao Lin, Mahmoud A. Ghannoum, Nancy Isham, Dan J. Diekema, Michael A. Pfaller, Shawn Messer, Shawn R. Lockhart, Naureen Iqbal, Vishnu Chaturvedi

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Four laboratories tested three isolates of Candida species and two isolates of Aspergillus fumigatus using 96-well plates containing combinations of amphotericin B, anidulafungin, caspofungin, micafungin, fluconazole, itraconazole, posaconazole, and voriconazole. The majority of summation fractional inhibitory concentration indices (ΣFICI) based on the Lowe additivity formula suggested indifferent drug interactions (ΣFICI>0.5 and≤4.0) and no instance of drug antagonism (ΣFICI>4.0). The intra-and interlaboratory agreement rates were superior when MIC₁₀₀ readings were used as endpoints (at a 99% confidence interval [CI]).

Original language	English (US)
Pages (from-to)	1759-1766
Number of pages	8
Journal	Antimicrobial agents and chemotherapy
Volume	59
Issue number	3
DOIs	https://doi.org/10.1128/AAC.04545-14
State	Published - Mar 1 2015
Externally published	Yes

ASJC Scopus subject areas

Pharmacology
Pharmacology (medical)
Infectious Diseases

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Ren, P., Luo, M., Lin, S., Ghannoum, M. A., Isham, N., Diekema, D. J., Pfaller, M. A., Messer, S., Lockhart, S. R., Iqbal, N., & Chaturvedi, V. (2015). Multilaboratory testing of antifungal drug combinations against Candida species and Aspergillus fumigatus: Utility of 100 percent inhibition as the endpoint. Antimicrobial agents and chemotherapy, 59(3), 1759-1766. https://doi.org/10.1128/AAC.04545-14

Ren, P, Luo, M, Lin, S, Ghannoum, MA, Isham, N, Diekema, DJ, Pfaller, MA, Messer, S, Lockhart, SR, Iqbal, N & Chaturvedi, V 2015, 'Multilaboratory testing of antifungal drug combinations against Candida species and Aspergillus fumigatus: Utility of 100 percent inhibition as the endpoint', Antimicrobial agents and chemotherapy, vol. 59, no. 3, pp. 1759-1766. https://doi.org/10.1128/AAC.04545-14

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AU - Isham, Nancy

AU - Diekema, Dan J.

AU - Pfaller, Michael A.

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Multilaboratory testing of antifungal drug combinations against Candida species and Aspergillus fumigatus: Utility of 100 percent inhibition as the endpoint

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