Multilaboratory testing of antifungal drug combinations against Candida species and Aspergillus fumigatus: Utility of 100 percent inhibition as the endpoint

Ping Ren, Ming Luo, Shao Lin, Mahmoud A. Ghannoum, Nancy Isham, Dan J. Diekema, Michael A. Pfaller, Shawn Messer, Shawn R. Lockhart, Naureen Iqbal, Vishnu Chaturvedi

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Four laboratories tested three isolates of Candida species and two isolates of Aspergillus fumigatus using 96-well plates containing combinations of amphotericin B, anidulafungin, caspofungin, micafungin, fluconazole, itraconazole, posaconazole, and voriconazole. The majority of summation fractional inhibitory concentration indices (ΣFICI) based on the Lowe additivity formula suggested indifferent drug interactions (ΣFICI>0.5 and≤4.0) and no instance of drug antagonism (ΣFICI>4.0). The intra-and interlaboratory agreement rates were superior when MIC100 readings were used as endpoints (at a 99% confidence interval [CI]).

Original languageEnglish (US)
Pages (from-to)1759-1766
Number of pages8
JournalAntimicrobial Agents and Chemotherapy
Volume59
Issue number3
DOIs
StatePublished - Mar 1 2015
Externally publishedYes

Fingerprint

caspofungin
anidulafungin
Drug Antagonism
Aspergillus fumigatus
Itraconazole
Fluconazole
Amphotericin B
Drug Combinations
Drug Interactions
Candida
Reading
Confidence Intervals
posaconazole
Voriconazole
micafungin

ASJC Scopus subject areas

  • Pharmacology
  • Pharmacology (medical)
  • Infectious Diseases

Cite this

Multilaboratory testing of antifungal drug combinations against Candida species and Aspergillus fumigatus : Utility of 100 percent inhibition as the endpoint. / Ren, Ping; Luo, Ming; Lin, Shao; Ghannoum, Mahmoud A.; Isham, Nancy; Diekema, Dan J.; Pfaller, Michael A.; Messer, Shawn; Lockhart, Shawn R.; Iqbal, Naureen; Chaturvedi, Vishnu.

In: Antimicrobial Agents and Chemotherapy, Vol. 59, No. 3, 01.03.2015, p. 1759-1766.

Research output: Contribution to journalArticle

Ren, P, Luo, M, Lin, S, Ghannoum, MA, Isham, N, Diekema, DJ, Pfaller, MA, Messer, S, Lockhart, SR, Iqbal, N & Chaturvedi, V 2015, 'Multilaboratory testing of antifungal drug combinations against Candida species and Aspergillus fumigatus: Utility of 100 percent inhibition as the endpoint', Antimicrobial Agents and Chemotherapy, vol. 59, no. 3, pp. 1759-1766. https://doi.org/10.1128/AAC.04545-14
Ren, Ping ; Luo, Ming ; Lin, Shao ; Ghannoum, Mahmoud A. ; Isham, Nancy ; Diekema, Dan J. ; Pfaller, Michael A. ; Messer, Shawn ; Lockhart, Shawn R. ; Iqbal, Naureen ; Chaturvedi, Vishnu. / Multilaboratory testing of antifungal drug combinations against Candida species and Aspergillus fumigatus : Utility of 100 percent inhibition as the endpoint. In: Antimicrobial Agents and Chemotherapy. 2015 ; Vol. 59, No. 3. pp. 1759-1766.
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