Multiple conformations of PEVK proteins detected by single-molecule techniques

Hongbin Li, Andres Oberhauser, Sambra D. Redick, Mariano Carrion-Vazquez, Harold P. Erickson, Julio M. Fernandez

Research output: Contribution to journalArticle

136 Citations (Scopus)

Abstract

An important component of muscle elasticity is the PEVK region of titin, so named because of the preponderance of these amino acids. However, the PEVK region, similar to other elastomeric proteins, is thought to form a random coil and therefore its structure cannot be determined by standard techniques. Here we combine single-molecule electron microscopy and atomic force microscopy to examine the conformations of the human cardiac titin PEVK region. In contrast to a simple random coil, we have found that cardiac PEVK shows a wide range of elastic conformations with end-to-end distances ranging from 9 to 24 nm and persistence lengths from 0.4 to 2.5 nm. Individual PEVK molecules retained their distinctive elastic conformations through many stretch-relaxation cycles, consistent with the view that these PEVK conformers cannot be interconverted by force. The multiple elastic conformations of cardiac PEVK may result from varying degrees of proline isomerization. The single-molecule techniques demonstrated here may help elucidate the conformation of other proteins that lack a well-defined structure.

Original languageEnglish (US)
Pages (from-to)10682-10686
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume98
Issue number19
DOIs
StatePublished - Sep 11 2001
Externally publishedYes

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Connectin
Protein Conformation
Atomic Force Microscopy
Elasticity
Proline
Electron Microscopy
Amino Acids
Muscles
Proteins
Single Molecule Imaging
elastomeric

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Multiple conformations of PEVK proteins detected by single-molecule techniques. / Li, Hongbin; Oberhauser, Andres; Redick, Sambra D.; Carrion-Vazquez, Mariano; Erickson, Harold P.; Fernandez, Julio M.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, No. 19, 11.09.2001, p. 10682-10686.

Research output: Contribution to journalArticle

Li, Hongbin ; Oberhauser, Andres ; Redick, Sambra D. ; Carrion-Vazquez, Mariano ; Erickson, Harold P. ; Fernandez, Julio M. / Multiple conformations of PEVK proteins detected by single-molecule techniques. In: Proceedings of the National Academy of Sciences of the United States of America. 2001 ; Vol. 98, No. 19. pp. 10682-10686.
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