Multiple glucocorticoid receptor transcripts in membrane glucocorticoid receptor-enriched S-49 mouse lymphoma cells

Fanghong Chen, Cheryl S. Watson, Bahiru Gametchu

    Research output: Contribution to journalArticle

    45 Citations (Scopus)

    Abstract

    A cDNA library from plasma membrane glucocorticoid receptor-enriched (mGR++) S- 49 mouse T lymphoma cells was screened with full-length rat intracellular GR (iGR) cDNA, BUGR-2 antibody, and PCR amplimers to portions of the mouse GR cDNA. One or two single-base substitutions resulting in amino acid changes (which do not incapacitate the receptor) were found in all but one clone: Val437 → Gly (located in the first zinc finger), and Glu546 → Gly (in the steroid-binding domain). Two previously unidentified exon 1 variants (1D and 1E), and two of three previously reported variants (1A, 1B) were found to be spliced onto the common exon 2. Exon 1D- and 1E-containing transcripts were confirmed by direct sequencing of amplimers from reverse transcriptase-coupled PCR. RNase protection studies revealed that one of these transcripts was expressed in mGR++ cells only, but not in two mGR- less (mGR-- S-49, and AtT-20 mouse pituitary) cell lines. These studies suggest that at least four promoters may be responsible for the control of GR (iGR and mGR) types in mouse lymphoma cells.

    Original languageEnglish (US)
    Pages (from-to)418-429
    Number of pages12
    JournalJournal of Cellular Biochemistry
    Volume74
    Issue number3
    DOIs
    StatePublished - Sep 1 1999

    Fingerprint

    Glucocorticoid Receptors
    Exons
    Lymphoma
    Membranes
    Complementary DNA
    RNA-Directed DNA Polymerase
    Cell membranes
    Ribonucleases
    Gene Library
    Zinc
    Rats
    T-Cell Lymphoma
    Zinc Fingers
    Substitution reactions
    Reverse Transcriptase Polymerase Chain Reaction
    Steroids
    Cells
    Amino Acids
    Antibodies
    Clone Cells

    Keywords

    • Alternative RNA splicing
    • Leukemia
    • Membrane glucocorticoid receptor
    • Polymerase chain reaction

    ASJC Scopus subject areas

    • Biochemistry
    • Cell Biology

    Cite this

    Multiple glucocorticoid receptor transcripts in membrane glucocorticoid receptor-enriched S-49 mouse lymphoma cells. / Chen, Fanghong; Watson, Cheryl S.; Gametchu, Bahiru.

    In: Journal of Cellular Biochemistry, Vol. 74, No. 3, 01.09.1999, p. 418-429.

    Research output: Contribution to journalArticle

    Chen, Fanghong ; Watson, Cheryl S. ; Gametchu, Bahiru. / Multiple glucocorticoid receptor transcripts in membrane glucocorticoid receptor-enriched S-49 mouse lymphoma cells. In: Journal of Cellular Biochemistry. 1999 ; Vol. 74, No. 3. pp. 418-429.
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