Multiple intrasplenic hepatocyte transplantations in the dalmatian dog

Ty B. Dunn, Norman H. Kumins, Vandad Raofi, Dawn M. Holman, Michael Mihalov, Jackie Blanchard, William R. Law, Cristiana Rastellini, Enrico Benedetti

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Background. Hepatocyte transplantation is an attractive potential treatment for liver-based inborn errors of metabolism and for fulminant hepatic failure. Dalmatian dogs have a metabolic error that results in hyperuricosuria. This report focuses on the effect of multiple, sequential intrasplenic transplants of fresh and cryopreserved hepatocytes in dalmatians. Methods. Dalmatians underwent intrasplenic hepatocyte transplantation with hepatocytes taken from healthy mongrels. Dalmatian urinary uric acid excretion was measured preoperatively, and this served as the control value. Three hepatocyte transplantations were performed at 30-day intervals-the first with freshly isolated cells, and both the second and the third with cryopreserved hepatocytes from the same donor. Urinary uric acid excretion was measured postoperatively twice per week. Results. The urinary uric acid excretion decreased an average of 54% after the first hepatocyte transplantation. The effect was transient and lasted an average of 22 days (range, 19-50 days). Subsequent intrasplenic hepatocyte transplantation with cryopreserved hepatocytes resulted in similar decreases in urinary uric acid excretion. Each transplant resulted in a significant decrease in urinary uric acid excretion when compared with baseline values (P = <.001). Conclusions. Sequential intrasplenic hepatocyte transplantation is feasible in this model. This method provided a significant, but transient, correction in urinary uric acid excretion that was similar with either fresh or cryopreserved hepatocytes. A substantial biologic effect provided by cryopreserved hepatocytes has important implications in clinical hepatocyte transplantation.

Original languageEnglish (US)
Pages (from-to)193-199
Number of pages7
JournalSurgery
Volume127
Issue number2
StatePublished - 2000
Externally publishedYes

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Hepatocytes
Transplantation
Dogs
Uric Acid
Transplants
Inborn Errors Metabolism
Acute Liver Failure
Liver

ASJC Scopus subject areas

  • Surgery

Cite this

Dunn, T. B., Kumins, N. H., Raofi, V., Holman, D. M., Mihalov, M., Blanchard, J., ... Benedetti, E. (2000). Multiple intrasplenic hepatocyte transplantations in the dalmatian dog. Surgery, 127(2), 193-199.

Multiple intrasplenic hepatocyte transplantations in the dalmatian dog. / Dunn, Ty B.; Kumins, Norman H.; Raofi, Vandad; Holman, Dawn M.; Mihalov, Michael; Blanchard, Jackie; Law, William R.; Rastellini, Cristiana; Benedetti, Enrico.

In: Surgery, Vol. 127, No. 2, 2000, p. 193-199.

Research output: Contribution to journalArticle

Dunn, TB, Kumins, NH, Raofi, V, Holman, DM, Mihalov, M, Blanchard, J, Law, WR, Rastellini, C & Benedetti, E 2000, 'Multiple intrasplenic hepatocyte transplantations in the dalmatian dog', Surgery, vol. 127, no. 2, pp. 193-199.
Dunn TB, Kumins NH, Raofi V, Holman DM, Mihalov M, Blanchard J et al. Multiple intrasplenic hepatocyte transplantations in the dalmatian dog. Surgery. 2000;127(2):193-199.
Dunn, Ty B. ; Kumins, Norman H. ; Raofi, Vandad ; Holman, Dawn M. ; Mihalov, Michael ; Blanchard, Jackie ; Law, William R. ; Rastellini, Cristiana ; Benedetti, Enrico. / Multiple intrasplenic hepatocyte transplantations in the dalmatian dog. In: Surgery. 2000 ; Vol. 127, No. 2. pp. 193-199.
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abstract = "Background. Hepatocyte transplantation is an attractive potential treatment for liver-based inborn errors of metabolism and for fulminant hepatic failure. Dalmatian dogs have a metabolic error that results in hyperuricosuria. This report focuses on the effect of multiple, sequential intrasplenic transplants of fresh and cryopreserved hepatocytes in dalmatians. Methods. Dalmatians underwent intrasplenic hepatocyte transplantation with hepatocytes taken from healthy mongrels. Dalmatian urinary uric acid excretion was measured preoperatively, and this served as the control value. Three hepatocyte transplantations were performed at 30-day intervals-the first with freshly isolated cells, and both the second and the third with cryopreserved hepatocytes from the same donor. Urinary uric acid excretion was measured postoperatively twice per week. Results. The urinary uric acid excretion decreased an average of 54{\%} after the first hepatocyte transplantation. The effect was transient and lasted an average of 22 days (range, 19-50 days). Subsequent intrasplenic hepatocyte transplantation with cryopreserved hepatocytes resulted in similar decreases in urinary uric acid excretion. Each transplant resulted in a significant decrease in urinary uric acid excretion when compared with baseline values (P = <.001). Conclusions. Sequential intrasplenic hepatocyte transplantation is feasible in this model. This method provided a significant, but transient, correction in urinary uric acid excretion that was similar with either fresh or cryopreserved hepatocytes. A substantial biologic effect provided by cryopreserved hepatocytes has important implications in clinical hepatocyte transplantation.",
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AU - Kumins, Norman H.

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AU - Mihalov, Michael

AU - Blanchard, Jackie

AU - Law, William R.

AU - Rastellini, Cristiana

AU - Benedetti, Enrico

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