Multiple pathways to the attenuation of West Nile virus in South-East Texas in 2003

Fiona J. May, Li Li, C. Todd Davis, Sareen E. Galbraith, Alan Barrett

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

West Nile virus (WNV) was first detected in Texas in 2002. During 2003, several isolates exhibiting significant attenuation of mouse neuroinvasiveness, and in some cases a small plaque and temperature sensitive phenotype when compared to other North American WNV isolates, were obtained from birds and mosquitoes in South-East Texas. To determine the attenuation markers of WNV, we have sequenced the genomes of three attenuated isolates and four temporally related virulent isolates and compared the amino acid substitutions in a total of 101 isolates, including three previously published genomes of attenuated strains, to identify mutations that are potentially involved in attenuation. Surprisingly, the attenuated strains fall into three separate "groups", suggesting that the attenuated phenotype evolved on three separate occasions in 2003. None of the groups share the same nucleotide changes or amino acid substitutions, and there are few mutations that can be clearly defined alone as being associated with attenuation.

Original languageEnglish (US)
Pages (from-to)8-14
Number of pages7
JournalVirology
Volume405
Issue number1
DOIs
StatePublished - Sep 2010

Fingerprint

West Nile virus
Amino Acid Substitution
Genome
Phenotype
Mutation
Culicidae
Birds
Nucleotides
Temperature

Keywords

  • Attenuation
  • Flaviviruses
  • West Nile virus

ASJC Scopus subject areas

  • Virology

Cite this

Multiple pathways to the attenuation of West Nile virus in South-East Texas in 2003. / May, Fiona J.; Li, Li; Davis, C. Todd; Galbraith, Sareen E.; Barrett, Alan.

In: Virology, Vol. 405, No. 1, 09.2010, p. 8-14.

Research output: Contribution to journalArticle

May, Fiona J. ; Li, Li ; Davis, C. Todd ; Galbraith, Sareen E. ; Barrett, Alan. / Multiple pathways to the attenuation of West Nile virus in South-East Texas in 2003. In: Virology. 2010 ; Vol. 405, No. 1. pp. 8-14.
@article{d1c0cf1bb7154e1b8845bd14b7bd8d4f,
title = "Multiple pathways to the attenuation of West Nile virus in South-East Texas in 2003",
abstract = "West Nile virus (WNV) was first detected in Texas in 2002. During 2003, several isolates exhibiting significant attenuation of mouse neuroinvasiveness, and in some cases a small plaque and temperature sensitive phenotype when compared to other North American WNV isolates, were obtained from birds and mosquitoes in South-East Texas. To determine the attenuation markers of WNV, we have sequenced the genomes of three attenuated isolates and four temporally related virulent isolates and compared the amino acid substitutions in a total of 101 isolates, including three previously published genomes of attenuated strains, to identify mutations that are potentially involved in attenuation. Surprisingly, the attenuated strains fall into three separate {"}groups{"}, suggesting that the attenuated phenotype evolved on three separate occasions in 2003. None of the groups share the same nucleotide changes or amino acid substitutions, and there are few mutations that can be clearly defined alone as being associated with attenuation.",
keywords = "Attenuation, Flaviviruses, West Nile virus",
author = "May, {Fiona J.} and Li Li and Davis, {C. Todd} and Galbraith, {Sareen E.} and Alan Barrett",
year = "2010",
month = "9",
doi = "10.1016/j.virol.2010.04.022",
language = "English (US)",
volume = "405",
pages = "8--14",
journal = "Virology",
issn = "0042-6822",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Multiple pathways to the attenuation of West Nile virus in South-East Texas in 2003

AU - May, Fiona J.

AU - Li, Li

AU - Davis, C. Todd

AU - Galbraith, Sareen E.

AU - Barrett, Alan

PY - 2010/9

Y1 - 2010/9

N2 - West Nile virus (WNV) was first detected in Texas in 2002. During 2003, several isolates exhibiting significant attenuation of mouse neuroinvasiveness, and in some cases a small plaque and temperature sensitive phenotype when compared to other North American WNV isolates, were obtained from birds and mosquitoes in South-East Texas. To determine the attenuation markers of WNV, we have sequenced the genomes of three attenuated isolates and four temporally related virulent isolates and compared the amino acid substitutions in a total of 101 isolates, including three previously published genomes of attenuated strains, to identify mutations that are potentially involved in attenuation. Surprisingly, the attenuated strains fall into three separate "groups", suggesting that the attenuated phenotype evolved on three separate occasions in 2003. None of the groups share the same nucleotide changes or amino acid substitutions, and there are few mutations that can be clearly defined alone as being associated with attenuation.

AB - West Nile virus (WNV) was first detected in Texas in 2002. During 2003, several isolates exhibiting significant attenuation of mouse neuroinvasiveness, and in some cases a small plaque and temperature sensitive phenotype when compared to other North American WNV isolates, were obtained from birds and mosquitoes in South-East Texas. To determine the attenuation markers of WNV, we have sequenced the genomes of three attenuated isolates and four temporally related virulent isolates and compared the amino acid substitutions in a total of 101 isolates, including three previously published genomes of attenuated strains, to identify mutations that are potentially involved in attenuation. Surprisingly, the attenuated strains fall into three separate "groups", suggesting that the attenuated phenotype evolved on three separate occasions in 2003. None of the groups share the same nucleotide changes or amino acid substitutions, and there are few mutations that can be clearly defined alone as being associated with attenuation.

KW - Attenuation

KW - Flaviviruses

KW - West Nile virus

UR - http://www.scopus.com/inward/record.url?scp=77955518877&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77955518877&partnerID=8YFLogxK

U2 - 10.1016/j.virol.2010.04.022

DO - 10.1016/j.virol.2010.04.022

M3 - Article

C2 - 20580395

AN - SCOPUS:77955518877

VL - 405

SP - 8

EP - 14

JO - Virology

JF - Virology

SN - 0042-6822

IS - 1

ER -