Multiplex analysis of cytokines in the blood of cynomolgus macaques naturally infected with Ebola virus (Reston serotype)

Karen L. Hutchinson, Francois Villinger, Mary Elizabeth Miranda, Thomas Ksiazek, C. J. Peters, Pierre E. Rollin

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

Ebola virus (EBO) causes the most severe form of viral hemorrhagic fever in humans and nonhuman primates with up to 90% of infections culminating in death. The requirement of maximum containment laboratories for Ebola virus research has limited opportunities to study the pathogenesis of EBO infections. While tissue damage does occur, often it would appear not to be sufficient to explain death, indicating that soluble mediators play an important role in disease progression. In previous studies, fatal human infections with the Zaire subtype of Ebola (EBO-Z) were associated with an increase in the levels of inflammatory cytokines. In this investigation, a new multiplex assay was developed and used to measure circulating levels of cytokines and chemokines in cynomolgus macaques infected with the Reston subtype of EBO (EBO-R). Increased levels of IL-6, TNF-α, IFN-γ, IL-2, IL-4, IL-8, IL-10, and GM-CSF were detected in infected animals, and the increase in circulating cytokines correlated with an increase in circulating viral antigen. Blood samples from animals showing high levels of cytokines were also tested for the chemokines: MCP-1, IL-1β, MIP-1α, MIP-lβ, IP-10, and RANTES. High levels of MCP-1 and MIP-1β, and RANTES were found in infected primates and, while levels were more variable, IL-1β was detected only in infected animals.

Original languageEnglish (US)
Pages (from-to)561-566
Number of pages6
JournalJournal of Medical Virology
Volume65
Issue number3
DOIs
StatePublished - 2001
Externally publishedYes

Fingerprint

Ebolavirus
Macaca
Cytokines
Chemokine CCL5
Interleukin-1
Chemokines
Primates
Ebola Hemorrhagic Fever
Viral Hemorrhagic Fevers
Democratic Republic of the Congo
Viral Antigens
Granulocyte-Macrophage Colony-Stimulating Factor
Infection
Interleukin-8
Interleukin-4
Interleukin-10
Interleukin-2
Disease Progression
Interleukin-6
Serogroup

Keywords

  • Chemokines
  • Hemorrhagic fever
  • Nonhuman primate
  • TNF α, IFN γ, IL-6

ASJC Scopus subject areas

  • Virology

Cite this

Multiplex analysis of cytokines in the blood of cynomolgus macaques naturally infected with Ebola virus (Reston serotype). / Hutchinson, Karen L.; Villinger, Francois; Miranda, Mary Elizabeth; Ksiazek, Thomas; Peters, C. J.; Rollin, Pierre E.

In: Journal of Medical Virology, Vol. 65, No. 3, 2001, p. 561-566.

Research output: Contribution to journalArticle

Hutchinson, Karen L. ; Villinger, Francois ; Miranda, Mary Elizabeth ; Ksiazek, Thomas ; Peters, C. J. ; Rollin, Pierre E. / Multiplex analysis of cytokines in the blood of cynomolgus macaques naturally infected with Ebola virus (Reston serotype). In: Journal of Medical Virology. 2001 ; Vol. 65, No. 3. pp. 561-566.
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