TY - JOUR
T1 - Murine and related chapparvoviruses are nephro-tropic and produce novel accessory proteins in infected kidneys
AU - Lee, Quintin
AU - Padula, Matthew P.
AU - Pinello, Natalia
AU - Williams, Simon H.
AU - O’Rourke, Matthew B.
AU - Fumagalli, Marcilio Jorge
AU - Orkin, Joseph D.
AU - Song, Renhua
AU - Shaban, Babak
AU - Brenner, Ori
AU - Pimanda, John E.
AU - Weninger, Wolfgang
AU - de Souza, William Marciel
AU - Melin, Amanda D.
AU - Wong, Justin J.L.
AU - Crim, Marcus J.
AU - Monette, Sébastien
AU - Roediger, Ben
AU - Jolly, Christopher J.
N1 - Publisher Copyright:
© 2020 Lee et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
PY - 2020
Y1 - 2020
N2 - Mouse kidney parvovirus (MKPV) is a member of the provisional genus Chapparvovirus that causes renal disease in immune-compromised mice, with a disease course reminiscent of polyomavirus-associated nephropathy in immune-suppressed kidney transplant patients. Here we map four major MKPV transcripts, created by alternative splicing, to a common initiator region, and use mass spectrometry to identify “p10” and “p15” as novel chapparvovirus accessory proteins produced in MKPV-infected kidneys. p15 and the splicing-dependent putative accessory protein NS2 are conserved in all near-complete amniote chapparvovirus genomes currently available (from mammals, birds and a reptile). In contrast, p10 may be encoded only by viruses with >60% amino acid identity to MKPV. We show that MKPV is kidney-tropic and that the bat chapparvovirus DrPV-1 and a non-human primate chapparvovirus, CKPV, are also found in the kidneys of their hosts. We propose, therefore, that many mammal chapparvoviruses are likely to be nephrotropic.
AB - Mouse kidney parvovirus (MKPV) is a member of the provisional genus Chapparvovirus that causes renal disease in immune-compromised mice, with a disease course reminiscent of polyomavirus-associated nephropathy in immune-suppressed kidney transplant patients. Here we map four major MKPV transcripts, created by alternative splicing, to a common initiator region, and use mass spectrometry to identify “p10” and “p15” as novel chapparvovirus accessory proteins produced in MKPV-infected kidneys. p15 and the splicing-dependent putative accessory protein NS2 are conserved in all near-complete amniote chapparvovirus genomes currently available (from mammals, birds and a reptile). In contrast, p10 may be encoded only by viruses with >60% amino acid identity to MKPV. We show that MKPV is kidney-tropic and that the bat chapparvovirus DrPV-1 and a non-human primate chapparvovirus, CKPV, are also found in the kidneys of their hosts. We propose, therefore, that many mammal chapparvoviruses are likely to be nephrotropic.
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U2 - 10.1371/journal.ppat.1008262
DO - 10.1371/journal.ppat.1008262
M3 - Article
C2 - 31971979
AN - SCOPUS:85078899294
SN - 1553-7366
VL - 16
JO - PLoS pathogens
JF - PLoS pathogens
IS - 1
M1 - e1008262
ER -