Murine coronavirus packaging signal confers packaging to nonviral RNA

Kangmee Woo, Myungsoo Joo, Krishna Narayanan, Kyongmin Hwang Kim, Shinji Makino

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Studies of defective interfering (DI) RNAs of the murine coronavirus mouse hepatitis virus (MHV) suggest that a 69-nucleotide-long packaging signal is necessary for MHV genomic RNA packaging into MHV particles. In this study we showed that when RNA transcripts that consisted of a non-MHV sequence and the packaging signal were expressed in MHV-infected cells, they were packaged into MHV particles. Those RNA transcripts that lacked the packaging signal or those containing a mutated packaging signal did not package efficiently. Thus, the presence of the packaging signal was sufficient for RNA packaging into MHV particles.

Original languageEnglish (US)
Pages (from-to)824-827
Number of pages4
JournalJournal of Virology
Volume71
Issue number1
StatePublished - 1997
Externally publishedYes

Fingerprint

Murine coronavirus
Murine hepatitis virus
Coronavirus
Product Packaging
packaging
RNA
virion
Virion
messenger RNA
Virus Assembly
Hepatitis Viruses
hepatitis
Protein Sorting Signals
Nucleotides
nucleotides
genomics
viruses

ASJC Scopus subject areas

  • Immunology

Cite this

Woo, K., Joo, M., Narayanan, K., Kim, K. H., & Makino, S. (1997). Murine coronavirus packaging signal confers packaging to nonviral RNA. Journal of Virology, 71(1), 824-827.

Murine coronavirus packaging signal confers packaging to nonviral RNA. / Woo, Kangmee; Joo, Myungsoo; Narayanan, Krishna; Kim, Kyongmin Hwang; Makino, Shinji.

In: Journal of Virology, Vol. 71, No. 1, 1997, p. 824-827.

Research output: Contribution to journalArticle

Woo, K, Joo, M, Narayanan, K, Kim, KH & Makino, S 1997, 'Murine coronavirus packaging signal confers packaging to nonviral RNA', Journal of Virology, vol. 71, no. 1, pp. 824-827.
Woo, Kangmee ; Joo, Myungsoo ; Narayanan, Krishna ; Kim, Kyongmin Hwang ; Makino, Shinji. / Murine coronavirus packaging signal confers packaging to nonviral RNA. In: Journal of Virology. 1997 ; Vol. 71, No. 1. pp. 824-827.
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