Murine coronavirus packaging signal confers packaging to nonviral RNA

Kangmee Woo, Myungsoo Joo, Krishna Narayanan, Kyongmin Hwang Kim, Shinji Makinno

Research output: Contribution to journalArticle

34 Scopus citations

Abstract

Studies of defective interfering (DI) RNAs of the murine coronavirus mouse hepatitis virus (MHV) suggest that a 69-nucleotide-long packaging signal is necessary for MHV genomic RNA packaging into MHV particles. In this study we showed that when RNA transcripts that consisted of a non-MHV sequence and the packaging signal were expressed in MHV-infected cells, they were packaged into MHV particles. Those RNA transcripts that lacked the packaging signal or those containing a mutated packaging signal did not package efficiently. Thus, the presence of the packaging signal was sufficient for RNA packaging into MHV particles.

Original languageEnglish (US)
Pages (from-to)824-827
Number of pages4
JournalJournal of virology
Volume71
Issue number1
DOIs
StatePublished - 1997

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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