Murine coronavirus packaging signal confers packaging to nonviral RNA

Kangmee Woo; Myungsoo Joo; Krishna Narayanan; Kyongmin Hwang Kim; Shinji Makinno

doi:10.1128/jvi.71.1.824-827.1997

Murine coronavirus packaging signal confers packaging to nonviral RNA

Kangmee Woo, Myungsoo Joo, Krishna Narayanan, Kyongmin Hwang Kim, Shinji Makinno

Research output: Contribution to journal › Article › peer-review

40 Scopus citations

Abstract

Studies of defective interfering (DI) RNAs of the murine coronavirus mouse hepatitis virus (MHV) suggest that a 69-nucleotide-long packaging signal is necessary for MHV genomic RNA packaging into MHV particles. In this study we showed that when RNA transcripts that consisted of a non-MHV sequence and the packaging signal were expressed in MHV-infected cells, they were packaged into MHV particles. Those RNA transcripts that lacked the packaging signal or those containing a mutated packaging signal did not package efficiently. Thus, the presence of the packaging signal was sufficient for RNA packaging into MHV particles.

Original language	English (US)
Pages (from-to)	824-827
Number of pages	4
Journal	Journal of virology
Volume	71
Issue number	1
DOIs	https://doi.org/10.1128/jvi.71.1.824-827.1997
State	Published - 1997
Externally published	Yes

ASJC Scopus subject areas

Microbiology
Immunology
Insect Science
Virology

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10.1128/jvi.71.1.824-827.1997

Cite this

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title = "Murine coronavirus packaging signal confers packaging to nonviral RNA",

abstract = "Studies of defective interfering (DI) RNAs of the murine coronavirus mouse hepatitis virus (MHV) suggest that a 69-nucleotide-long packaging signal is necessary for MHV genomic RNA packaging into MHV particles. In this study we showed that when RNA transcripts that consisted of a non-MHV sequence and the packaging signal were expressed in MHV-infected cells, they were packaged into MHV particles. Those RNA transcripts that lacked the packaging signal or those containing a mutated packaging signal did not package efficiently. Thus, the presence of the packaging signal was sufficient for RNA packaging into MHV particles.",

author = "Kangmee Woo and Myungsoo Joo and Krishna Narayanan and Kim, \{Kyongmin Hwang\} and Shinji Makinno",

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AU - Woo, Kangmee

AU - Joo, Myungsoo

AU - Narayanan, Krishna

AU - Kim, Kyongmin Hwang

AU - Makinno, Shinji

PY - 1997

Y1 - 1997

N2 - Studies of defective interfering (DI) RNAs of the murine coronavirus mouse hepatitis virus (MHV) suggest that a 69-nucleotide-long packaging signal is necessary for MHV genomic RNA packaging into MHV particles. In this study we showed that when RNA transcripts that consisted of a non-MHV sequence and the packaging signal were expressed in MHV-infected cells, they were packaged into MHV particles. Those RNA transcripts that lacked the packaging signal or those containing a mutated packaging signal did not package efficiently. Thus, the presence of the packaging signal was sufficient for RNA packaging into MHV particles.

AB - Studies of defective interfering (DI) RNAs of the murine coronavirus mouse hepatitis virus (MHV) suggest that a 69-nucleotide-long packaging signal is necessary for MHV genomic RNA packaging into MHV particles. In this study we showed that when RNA transcripts that consisted of a non-MHV sequence and the packaging signal were expressed in MHV-infected cells, they were packaged into MHV particles. Those RNA transcripts that lacked the packaging signal or those containing a mutated packaging signal did not package efficiently. Thus, the presence of the packaging signal was sufficient for RNA packaging into MHV particles.

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SP - 824

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JO - Journal of virology

JF - Journal of virology

IS - 1

ER -

Murine coronavirus packaging signal confers packaging to nonviral RNA

Abstract

ASJC Scopus subject areas

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