Murine neutrophils require α 1,3-fucosylation but not PSGL-1 for productive infection with Anaplasma phagocytophilum

Jason A. Carlyon, Mustafa Akkoyunlu, Lijun Xia, Tadayuki Yago, Tian Wang, Richard D. Cummings, Rodger P. McEver, Erol Fikrig

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Anaplasma phagocytophilum causes human granulocytic ehrlichiosis, the second most common tick-borne disease in the United States. Mice are natural reservoirs for this bacterium and man is an inadvertent host. A phagocytophilum's tropism for human neutrophils is linked to neutrophil expression of P-selectin glycoprotein ligand-1 (PSGL-1), as well as sialylated and α1,3-fucosylated glycans. To determine whether A phagocytophilum uses similar molecular features to infect murine neutrophils, we assessed in vitro bacterial binding to neutrophils from and infection burden in wild-type mice; mice lacking α1,3-fucosyltransferases Fuc-TIV and Fuc-TVII; or mice lacking PSGL-1. Binding to Fuc-TIV-/-/Fuc-TVII-/- neutrophils and infection of Fuc-TIV-/-/Fuc-TVII-/- mice were significantly reduced relative to wild-type mice. A phagocytophilum binding to PSGL-1-/- neutrophils was modestly reduced, whereas sialidase treatment significantly decreased binding to both wild-type and PSGL-1-/- neutrophils. A phagocytophilum similarly infected PSGL-1-/- and wild-type mice in vivo. A phagocytophilum induced comparable levels of chemokines from wild-type and PSGL-1-/- neutrophils in vitro, while those induced from Fuc-TIV -/-/Fuc-TVII-/- neutrophils were appreciably reduced. Therefore, A phagocytophilum infection in mice, as in humans, requires sialylation and α1,3-fucosylation of neutrophils. However, murine infection does not require neutrophil PSGL-1 expression, which has important implications for understanding how A phagocytophilum binds and infects neutrophils.

Original languageEnglish (US)
Pages (from-to)3387-3395
Number of pages9
JournalBlood
Volume102
Issue number9
DOIs
StatePublished - Nov 1 2003
Externally publishedYes

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Anaplasma phagocytophilum
Neutrophils
Infection
galactoside 3-fucosyltransferase
Neuraminidase
P-selectin ligand protein
Chemokines
Polysaccharides
Bacteria
Ehrlichiosis
Tick-Borne Diseases
Tropism

ASJC Scopus subject areas

  • Hematology

Cite this

Murine neutrophils require α 1,3-fucosylation but not PSGL-1 for productive infection with Anaplasma phagocytophilum. / Carlyon, Jason A.; Akkoyunlu, Mustafa; Xia, Lijun; Yago, Tadayuki; Wang, Tian; Cummings, Richard D.; McEver, Rodger P.; Fikrig, Erol.

In: Blood, Vol. 102, No. 9, 01.11.2003, p. 3387-3395.

Research output: Contribution to journalArticle

Carlyon, JA, Akkoyunlu, M, Xia, L, Yago, T, Wang, T, Cummings, RD, McEver, RP & Fikrig, E 2003, 'Murine neutrophils require α 1,3-fucosylation but not PSGL-1 for productive infection with Anaplasma phagocytophilum', Blood, vol. 102, no. 9, pp. 3387-3395. https://doi.org/10.1182/blood-2003-02-0621
Carlyon, Jason A. ; Akkoyunlu, Mustafa ; Xia, Lijun ; Yago, Tadayuki ; Wang, Tian ; Cummings, Richard D. ; McEver, Rodger P. ; Fikrig, Erol. / Murine neutrophils require α 1,3-fucosylation but not PSGL-1 for productive infection with Anaplasma phagocytophilum. In: Blood. 2003 ; Vol. 102, No. 9. pp. 3387-3395.
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AU - Cummings, Richard D.

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N2 - Anaplasma phagocytophilum causes human granulocytic ehrlichiosis, the second most common tick-borne disease in the United States. Mice are natural reservoirs for this bacterium and man is an inadvertent host. A phagocytophilum's tropism for human neutrophils is linked to neutrophil expression of P-selectin glycoprotein ligand-1 (PSGL-1), as well as sialylated and α1,3-fucosylated glycans. To determine whether A phagocytophilum uses similar molecular features to infect murine neutrophils, we assessed in vitro bacterial binding to neutrophils from and infection burden in wild-type mice; mice lacking α1,3-fucosyltransferases Fuc-TIV and Fuc-TVII; or mice lacking PSGL-1. Binding to Fuc-TIV-/-/Fuc-TVII-/- neutrophils and infection of Fuc-TIV-/-/Fuc-TVII-/- mice were significantly reduced relative to wild-type mice. A phagocytophilum binding to PSGL-1-/- neutrophils was modestly reduced, whereas sialidase treatment significantly decreased binding to both wild-type and PSGL-1-/- neutrophils. A phagocytophilum similarly infected PSGL-1-/- and wild-type mice in vivo. A phagocytophilum induced comparable levels of chemokines from wild-type and PSGL-1-/- neutrophils in vitro, while those induced from Fuc-TIV -/-/Fuc-TVII-/- neutrophils were appreciably reduced. Therefore, A phagocytophilum infection in mice, as in humans, requires sialylation and α1,3-fucosylation of neutrophils. However, murine infection does not require neutrophil PSGL-1 expression, which has important implications for understanding how A phagocytophilum binds and infects neutrophils.

AB - Anaplasma phagocytophilum causes human granulocytic ehrlichiosis, the second most common tick-borne disease in the United States. Mice are natural reservoirs for this bacterium and man is an inadvertent host. A phagocytophilum's tropism for human neutrophils is linked to neutrophil expression of P-selectin glycoprotein ligand-1 (PSGL-1), as well as sialylated and α1,3-fucosylated glycans. To determine whether A phagocytophilum uses similar molecular features to infect murine neutrophils, we assessed in vitro bacterial binding to neutrophils from and infection burden in wild-type mice; mice lacking α1,3-fucosyltransferases Fuc-TIV and Fuc-TVII; or mice lacking PSGL-1. Binding to Fuc-TIV-/-/Fuc-TVII-/- neutrophils and infection of Fuc-TIV-/-/Fuc-TVII-/- mice were significantly reduced relative to wild-type mice. A phagocytophilum binding to PSGL-1-/- neutrophils was modestly reduced, whereas sialidase treatment significantly decreased binding to both wild-type and PSGL-1-/- neutrophils. A phagocytophilum similarly infected PSGL-1-/- and wild-type mice in vivo. A phagocytophilum induced comparable levels of chemokines from wild-type and PSGL-1-/- neutrophils in vitro, while those induced from Fuc-TIV -/-/Fuc-TVII-/- neutrophils were appreciably reduced. Therefore, A phagocytophilum infection in mice, as in humans, requires sialylation and α1,3-fucosylation of neutrophils. However, murine infection does not require neutrophil PSGL-1 expression, which has important implications for understanding how A phagocytophilum binds and infects neutrophils.

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