Murine T-helper cell immune response to recombinant vaccinia-Venezuelan equine encephalitis virus

James H. Mathews, Richard M. Kinney, John T. Roehrig, Alan D.T. Barrett, Dennis W. Trent

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

The T-helper (Th) cell immune response following immunization of C3H (H-2k) mice with a recombinant vaccinia (VAC) virus (TC-5A) expressing the structural proteins (capsid, E1 and E2) of the attenuated vaccine strain (TC-83) of Venezuelan equine encephalitis (VEE) virus was compared with the immune response induced in mice after immunization with TC-83 virus. TC-5A virus elicited Th cells that strongly recognized both VAC and TC-83 viruses in in vitro lymphoblastogenesis tests. Th-cell activation was associated with elevated levels of interleukin-2. TC-5A virus induced long-term humoral immunity; VEE virus-binding and neutralizing antibodies were detected in mouse sera collected from mice 16 months after a single immunization.

Original languageEnglish (US)
Pages (from-to)620-624
Number of pages5
JournalVaccine
Volume12
Issue number7
DOIs
StatePublished - 1994

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Keywords

  • Recombinant VAC-VEE virus
  • T-helper cell
  • immune response

ASJC Scopus subject areas

  • Molecular Medicine
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

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