Muscarinic acetylcholine receptor subtype expression in avian vestibular hair cells, nerve terminals and ganglion cells

G. Q. Li, G. A. Kevetter, R. B. Leonard, D. J. Prusak, Thomas Wood, M. J. Correia

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Muscarinic acetylcholine receptors (mAChRs) are widely expressed in the CNS and peripheral nervous system and play an important role in modulating the cell activity and function. We have shown that the cholinergic agonist carbachol reduces the pigeon's inwardly rectifying potassium channel (pKir2.1) ionic currents in native vestibular hair cells. We have cloned and sequenced pigeon mAChR subtypes M2-M5 and we have studied the expression of all five mAChR subtypes (M1-M5) in the pigeon vestibular end organs (semicircular canal ampullary cristae and utricular maculae), vestibular nerve fibers and the vestibular (Scarpa's) ganglion using tissue immunohistochemistry (IH), dissociated single cell immunocytochemistry (IC) and Western blotting (WB). We found that vestibular hair cells, nerve fibers and ganglion cells each expressed all five (M1-M5) mAChR subtypes. Two of the three odd-numbered mAChRs (M1, M5) were present on the hair cell cilia, supporting cells and nerve terminals. And all three odd numbered mAChRs (M1, M3 and M5) were expressed on cuticular plates, myelin sheaths and Schwann cells. Even-numbered mAChRs were seen on the nerve terminals. M2 was also shown on the cuticular plates and supporting cells. Vestibular efferent fibers and terminals were not identified in our studies. Results from WB of the dissociated vestibular epithelia, nerve fibers and vestibular ganglia were consistent with the results from IH and IC. Our findings suggest that there is considerable co-expression of the subtypes on the neural elements of the labyrinth. Further electrophysiological and pharmacological studies should delineate the mechanisms of action of muscarinic acetylcholine receptors on structures in the labyrinth.

Original languageEnglish (US)
Pages (from-to)384-402
Number of pages19
JournalNeuroscience
Volume146
Issue number1
DOIs
StatePublished - Apr 25 2007

Fingerprint

Vestibular Hair Cells
Muscarinic Receptors
Ganglia
Vestibular Nerve
Columbidae
Immunohistochemistry
Nerve Fibers
Muscarinic M1 Receptors
Inner Ear
Muscarinic M5 Receptors
Western Blotting
Inwardly Rectifying Potassium Channel
Cholinergic Agonists
Semicircular Canals
Cilia
Schwann Cells
Peripheral Nervous System
Carbachol
Myelin Sheath
Epithelium

Keywords

  • avian
  • G-protein
  • immunocytochemistry
  • immunohistochemistry
  • vestibular peripheral system
  • Western blotting

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Muscarinic acetylcholine receptor subtype expression in avian vestibular hair cells, nerve terminals and ganglion cells. / Li, G. Q.; Kevetter, G. A.; Leonard, R. B.; Prusak, D. J.; Wood, Thomas; Correia, M. J.

In: Neuroscience, Vol. 146, No. 1, 25.04.2007, p. 384-402.

Research output: Contribution to journalArticle

Li, G. Q. ; Kevetter, G. A. ; Leonard, R. B. ; Prusak, D. J. ; Wood, Thomas ; Correia, M. J. / Muscarinic acetylcholine receptor subtype expression in avian vestibular hair cells, nerve terminals and ganglion cells. In: Neuroscience. 2007 ; Vol. 146, No. 1. pp. 384-402.
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