Muscarinic stimulation of gallbladder epithelium I. Electrophysiology and signaling mechanisms

G. A. Altenberg, M. Subramanyam, J. S. Bergmann, K. M. Johnson, L. Reuss

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

To understand the effects of acetylcholine (ACh) on fluid-absorbing epithelia, we carried out experiments on Necturus gallbladder epithelium. Binding studies with 1-quinuclidinyl[phenyl-4(N)-3H]benzilate (QNB) demonstrated that Necturus gallbladder epithelial cells express high-affinity muscarinic receptors. The effects of ACh and carbachol were exerted from the basolateral surface and consisted of a transient hyperpolarization of both cell membranes and a concomitant decrease in the apparent fractional resistance of the apical membrane. Atropine blocked both effects. ACh also elicited transient elevations of inositol 1,4,5-trisphosphate and intracellular free calcium ([Ca2+](i)) levels, the latter by both release from intracellular stores and basolateral influx. The phospholipase C antagonist U-73122 inhibited the effects of ACh, whereas inhibition of prostaglandin and guanosine 3',5'-cyclic monophosphate synthesis with indomethacin or methylene blue, respectively, had no effect. In conclusion, Necturus gallbladder epithelium expresses muscarinic receptors in the basolateral membrane. Receptor activation stimulates phospholipase C and elevates cellular levels of inositol 1,4,5-trisphosphate and [Ca2+](i). The elevation in [Ca2+](i) activates K+ channels but apparently not Cl- channels.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Cell Physiology
Volume265
Issue number6 34-6
StatePublished - 1993

Fingerprint

Electrophysiology
Necturus
Gallbladder
Cholinergic Agents
Acetylcholine
Epithelium
Inositol 1,4,5-Trisphosphate
Type C Phospholipases
Muscarinic Receptors
Benzilates
Membranes
Methylene Blue
Cyclic GMP
Carbachol
Cell membranes
Atropine
Indomethacin
Prostaglandins
Epithelial Cells
Chemical activation

Keywords

  • acetylcholine
  • inositol 1,4,5-trisphosphate
  • intracellular calcium
  • maxi potassium channel
  • phospholipase C
  • proximal tubule
  • small intestine

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

Cite this

Altenberg, G. A., Subramanyam, M., Bergmann, J. S., Johnson, K. M., & Reuss, L. (1993). Muscarinic stimulation of gallbladder epithelium I. Electrophysiology and signaling mechanisms. American Journal of Physiology - Cell Physiology, 265(6 34-6).

Muscarinic stimulation of gallbladder epithelium I. Electrophysiology and signaling mechanisms. / Altenberg, G. A.; Subramanyam, M.; Bergmann, J. S.; Johnson, K. M.; Reuss, L.

In: American Journal of Physiology - Cell Physiology, Vol. 265, No. 6 34-6, 1993.

Research output: Contribution to journalArticle

Altenberg, GA, Subramanyam, M, Bergmann, JS, Johnson, KM & Reuss, L 1993, 'Muscarinic stimulation of gallbladder epithelium I. Electrophysiology and signaling mechanisms', American Journal of Physiology - Cell Physiology, vol. 265, no. 6 34-6.
Altenberg, G. A. ; Subramanyam, M. ; Bergmann, J. S. ; Johnson, K. M. ; Reuss, L. / Muscarinic stimulation of gallbladder epithelium I. Electrophysiology and signaling mechanisms. In: American Journal of Physiology - Cell Physiology. 1993 ; Vol. 265, No. 6 34-6.
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