Muscle-specific microRNA miR-206 promotes muscle differentiation

Kyun Kim Hak, Sun Lee Yong, Umasundari Sivaprasad, Ankit Malhotra, Anindya Dutta

Research output: Contribution to journalArticle

526 Citations (Scopus)

Abstract

Three muscle-specific microRNAs, miR-206, -1, and -133, are induced during differentiation of C2C12 myoblasts in vitro. Transfection of miR-206 promotes differentiation despite the presence of serum, whereas inhibition of the microRNA by antisense oligonucleotide inhibits cell cycle withdrawal and differentiation, which are normally induced by serum deprivation. Among the many mRNAs that are down-regulated by miR-206, the p180 subunit of DNA polymerase a and three other genes are shown to be direct targets. Down-regulation of the polymerase inhibits DNA synthesis, an important component of the differentiation program. The direct targets are decreased by mRNA cleavage that is dependent on predicted microRNA target sites. Unlike small interfering RNA-directed cleavage, however, the 5′ ends of the cleavage fragments are distributed and not confined to the target sites, suggesting involvement of exonucleases in the degradation process. In addition, inhibitors of myogenic transcription factors, Id1-3 and MyoR, are decreased upon miR-206 introduction, suggesting the presence of additional mechanisms by which microRNAs enforce the differentiation program.

Original languageEnglish (US)
Pages (from-to)677-687
Number of pages11
JournalJournal of Cell Biology
Volume174
Issue number5
DOIs
StatePublished - Aug 28 2006
Externally publishedYes

Fingerprint

MicroRNAs
Muscles
DNA-Directed DNA Polymerase
Transcription Factor 3
RNA Cleavage
Exonucleases
Messenger RNA
Antisense Oligonucleotides
Myoblasts
Serum
Small Interfering RNA
Transfection
Cell Differentiation
Cell Cycle
Down-Regulation
Genes

ASJC Scopus subject areas

  • Cell Biology

Cite this

Hak, K. K., Yong, S. L., Sivaprasad, U., Malhotra, A., & Dutta, A. (2006). Muscle-specific microRNA miR-206 promotes muscle differentiation. Journal of Cell Biology, 174(5), 677-687. https://doi.org/10.1083/jcb.200603008

Muscle-specific microRNA miR-206 promotes muscle differentiation. / Hak, Kyun Kim; Yong, Sun Lee; Sivaprasad, Umasundari; Malhotra, Ankit; Dutta, Anindya.

In: Journal of Cell Biology, Vol. 174, No. 5, 28.08.2006, p. 677-687.

Research output: Contribution to journalArticle

Hak, KK, Yong, SL, Sivaprasad, U, Malhotra, A & Dutta, A 2006, 'Muscle-specific microRNA miR-206 promotes muscle differentiation', Journal of Cell Biology, vol. 174, no. 5, pp. 677-687. https://doi.org/10.1083/jcb.200603008
Hak KK, Yong SL, Sivaprasad U, Malhotra A, Dutta A. Muscle-specific microRNA miR-206 promotes muscle differentiation. Journal of Cell Biology. 2006 Aug 28;174(5):677-687. https://doi.org/10.1083/jcb.200603008
Hak, Kyun Kim ; Yong, Sun Lee ; Sivaprasad, Umasundari ; Malhotra, Ankit ; Dutta, Anindya. / Muscle-specific microRNA miR-206 promotes muscle differentiation. In: Journal of Cell Biology. 2006 ; Vol. 174, No. 5. pp. 677-687.
@article{d1b4c39a74d646efb28cdca7f86e9783,
title = "Muscle-specific microRNA miR-206 promotes muscle differentiation",
abstract = "Three muscle-specific microRNAs, miR-206, -1, and -133, are induced during differentiation of C2C12 myoblasts in vitro. Transfection of miR-206 promotes differentiation despite the presence of serum, whereas inhibition of the microRNA by antisense oligonucleotide inhibits cell cycle withdrawal and differentiation, which are normally induced by serum deprivation. Among the many mRNAs that are down-regulated by miR-206, the p180 subunit of DNA polymerase a and three other genes are shown to be direct targets. Down-regulation of the polymerase inhibits DNA synthesis, an important component of the differentiation program. The direct targets are decreased by mRNA cleavage that is dependent on predicted microRNA target sites. Unlike small interfering RNA-directed cleavage, however, the 5′ ends of the cleavage fragments are distributed and not confined to the target sites, suggesting involvement of exonucleases in the degradation process. In addition, inhibitors of myogenic transcription factors, Id1-3 and MyoR, are decreased upon miR-206 introduction, suggesting the presence of additional mechanisms by which microRNAs enforce the differentiation program.",
author = "Hak, {Kyun Kim} and Yong, {Sun Lee} and Umasundari Sivaprasad and Ankit Malhotra and Anindya Dutta",
year = "2006",
month = "8",
day = "28",
doi = "10.1083/jcb.200603008",
language = "English (US)",
volume = "174",
pages = "677--687",
journal = "Journal of Cell Biology",
issn = "0021-9525",
publisher = "Rockefeller University Press",
number = "5",

}

TY - JOUR

T1 - Muscle-specific microRNA miR-206 promotes muscle differentiation

AU - Hak, Kyun Kim

AU - Yong, Sun Lee

AU - Sivaprasad, Umasundari

AU - Malhotra, Ankit

AU - Dutta, Anindya

PY - 2006/8/28

Y1 - 2006/8/28

N2 - Three muscle-specific microRNAs, miR-206, -1, and -133, are induced during differentiation of C2C12 myoblasts in vitro. Transfection of miR-206 promotes differentiation despite the presence of serum, whereas inhibition of the microRNA by antisense oligonucleotide inhibits cell cycle withdrawal and differentiation, which are normally induced by serum deprivation. Among the many mRNAs that are down-regulated by miR-206, the p180 subunit of DNA polymerase a and three other genes are shown to be direct targets. Down-regulation of the polymerase inhibits DNA synthesis, an important component of the differentiation program. The direct targets are decreased by mRNA cleavage that is dependent on predicted microRNA target sites. Unlike small interfering RNA-directed cleavage, however, the 5′ ends of the cleavage fragments are distributed and not confined to the target sites, suggesting involvement of exonucleases in the degradation process. In addition, inhibitors of myogenic transcription factors, Id1-3 and MyoR, are decreased upon miR-206 introduction, suggesting the presence of additional mechanisms by which microRNAs enforce the differentiation program.

AB - Three muscle-specific microRNAs, miR-206, -1, and -133, are induced during differentiation of C2C12 myoblasts in vitro. Transfection of miR-206 promotes differentiation despite the presence of serum, whereas inhibition of the microRNA by antisense oligonucleotide inhibits cell cycle withdrawal and differentiation, which are normally induced by serum deprivation. Among the many mRNAs that are down-regulated by miR-206, the p180 subunit of DNA polymerase a and three other genes are shown to be direct targets. Down-regulation of the polymerase inhibits DNA synthesis, an important component of the differentiation program. The direct targets are decreased by mRNA cleavage that is dependent on predicted microRNA target sites. Unlike small interfering RNA-directed cleavage, however, the 5′ ends of the cleavage fragments are distributed and not confined to the target sites, suggesting involvement of exonucleases in the degradation process. In addition, inhibitors of myogenic transcription factors, Id1-3 and MyoR, are decreased upon miR-206 introduction, suggesting the presence of additional mechanisms by which microRNAs enforce the differentiation program.

UR - http://www.scopus.com/inward/record.url?scp=33748102321&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33748102321&partnerID=8YFLogxK

U2 - 10.1083/jcb.200603008

DO - 10.1083/jcb.200603008

M3 - Article

VL - 174

SP - 677

EP - 687

JO - Journal of Cell Biology

JF - Journal of Cell Biology

SN - 0021-9525

IS - 5

ER -